Melvin Ezell Email & Phone Number

Rockville, Md

Rockville, Md

Maintained the responsibilities of an Associate scientist I Revised outdated documents to make them more coherent and concise Assisted Molecular development services to validate and transfer new assays and equipment to the Molecular departmentTrained new staff members on GMP & GLP regulated assaysContributed ideas and suggestions to improve standard laboratory techniques, processes, and equipmentOperates and maintained lab equipment as required by SOPs lab proceduresPerform DNA extraction and qPCR by using automation platforms such as the QIAcube and QIAgility. Use Nanodrop 8000 to evaluate nucleic acids for both quantity and purity after an extraction material

Performed safety testing for of biopharmaceutical products at different phases of clinical trials, cell lines, bulk lots as well as final products using several assays. Perform testing in accordance with SOPs regulations (cGMP and GLP)Perform quality check of the data using the Sequence Analysis Software. Perform qPCR to detect and quantify residual DNA or Viral RNA clearance in test articles. Prepare and qualify assay specific reagents for various assays. Extracted DNA and RNA using various kits.Quantify nucleic acids by: Picogreen, Ribogreen, and spectrometryExperience working in a BSL2 laboratory environmentMaintain complete and comprehensive records for study integrity including controlled documents, laboratory records, and equipment addendum.

Maintain health of mice; checking for unnecessary stress, proper levels of food and water, checking for flooding and cleanliness, log temperature and humidity of environmentPerformed anesthesia and euthanasia on mice

Perform radiation wipe test monthlyPrepare buffer solutions, clean and maintain glassware, create spreadsheets for order supplies using Microsoft ExcelPrepare slides for tissue sectioning; refill and maintain liquid nitrogen supply

Created in-frame deletions of 14 genes from the glycoside hydrolase family 43 in C. japonicus, to determine the target substrate of each of the GH43 genes. Utilized fundamental laboratory techniques including; Gibson assembly based cloning, primer design, PCR, cloning, sequencing Collaborated with two peers to research functional analysis of glycosylhydrolase Class 43 in C. japonicus, with two peers Conducted bi-monthly presentations to faculty and peers regarding progress of research Created poster displaying results of research; presented to undergrads and facility at GABS Produced non-thesis research paper on functional analysis of glycosylhydrolase Class 43 in Cellvibrio japoniucs: awaiting publicationMaintained an organized and up to date notebook of experimental procedures throughout the year

Performed radiation wipe test monthlyPrepared solutions, cleaned and maintained glassware

Master'S Degree, Applied Molecular Biology, 3.1

Ba, Biological Science