Mengdi Lou Email & Phone Number

San Francisco Bay Area

Oceanside, California, United States

Technology Application• Facilitated system transfer of Cytiva Wave25 from UNICORN to DeltaV software and trained end-users• Transferred the first concentrated fed-batch perfusion process in-house, coordinated with Automation group to guarantee appropriate equipment capability while working in lab to ensure operational robustness• Identified operational knowledge gaps and improved maintenance strategies for 4 Ambr250 systems • Led lab management efforts for calibration and… Show more Technology Application• Facilitated system transfer of Cytiva Wave25 from UNICORN to DeltaV software and trained end-users• Transferred the first concentrated fed-batch perfusion process in-house, coordinated with Automation group to guarantee appropriate equipment capability while working in lab to ensure operational robustness• Identified operational knowledge gaps and improved maintenance strategies for 4 Ambr250 systems • Led lab management efforts for calibration and preventative maintenance of key bioanalyzers• Developed PI Vision templates process visualization for across multiple reactor types (bench, ambr250)• Provided technical training and professional mentorship to 8 entry-level new hires, enabling them to lead experimental study execution within the first year of onboardingPipeline Development• Designed and led DOEs to determine the effects of process parameters on cell performance across scales• Identified raw material impurity as the source of poor performance in commercial manufacturing and led experiments justifying material changes that resulted in 40% titer recovery• Led MSAT experiments that resolved gaps between qualified small-scale model and at-scale manufacturing• Authored process characterization summary reports, reviewed batch records for a late-phase process transfer• Supported bench-scale satellite operation for clinical manufacturing of early-stage pipeline project• Communicated experimental findings and recommendations to upper management, including SVP-level Show less

Greater Minneapolis-St. Paul Area

Contributed to research in the Hu group at UMNTC in the field of cellular engineeringDeveloped purification protocol for separation of HIV Envelope trimer protein from protein aggregatesPerformed fed-batch cell culture and purification to obtain SARS-CoV2 Spike proteinDeveloped ELISA protocol for engineered Spike proteinAnalyzed purity of Spike protein product and identified protein aggregation issue along with potential solutionsPerformed MFA on GS CHO cell culture data and… Show more Contributed to research in the Hu group at UMNTC in the field of cellular engineeringDeveloped purification protocol for separation of HIV Envelope trimer protein from protein aggregatesPerformed fed-batch cell culture and purification to obtain SARS-CoV2 Spike proteinDeveloped ELISA protocol for engineered Spike proteinAnalyzed purity of Spike protein product and identified protein aggregation issue along with potential solutionsPerformed MFA on GS CHO cell culture data and identified environmental pH effects on cell metabolismExperimented with new bioreactor configuration for fed-batch cell culture using LabView for systems controlExamined the protein-processing abilities of the Golgi apparatus within mammalian cells to identify characteristics for enhancement of glycan profiles of therapeutic proteinsProficient in mammalian cell culture, bacterial cell culture, ELISA, affinity chromatography, elution chromatography, PAGE, isoelectric focusing, cell staining Show less

Investigated relationship between viral outcome and medical retainment of HIV+ case management clientsCollaborated with the Youth and AIDS Projects in Minneapolis, MN to organize and analyze relevant client dataOperated under the guidance of Jonathan Ravdin, MD

Oceanside, Ca

Designed and executed experiment varying media component concentrations with multiple cell lines Programmed and operated automated microbioreactor cell culture system, Sartorius ambr250Passaged cell seed train aseptically to support bioreactor experimentsPerformed HPLC data analysis through Chromeleon and visualized data in SpotfireLed vendor instrument comparison and presented statistical findings to managementEffectively communicated project results and significance at the… Show more Designed and executed experiment varying media component concentrations with multiple cell lines Programmed and operated automated microbioreactor cell culture system, Sartorius ambr250Passaged cell seed train aseptically to support bioreactor experimentsPerformed HPLC data analysis through Chromeleon and visualized data in SpotfireLed vendor instrument comparison and presented statistical findings to managementEffectively communicated project results and significance at the concluding intern presentation seminars Show less

Greater Minneapolis-St. Paul Area

Evaluated protein passivation capabilities of copolymer prototype for use in immunoassay buffersDesigned Ouchterlony double-immunodiffusion procedure for detection of immune complexes

Greater Minneapolis-St. Paul Area

Worked closely with physicians in a fast-paced, time sensitive emergency department setting.Closely shadowed the clinicians and efficiently handled time consuming clerical responsibilities during a patient's department encounter. Accurately documented encounter events and decision making in a manner that is appropriate for medical charting. Gained extensive clinical experience in a high intensity medical environment

Eden Prairie

Introduced math concepts to multiple students at a timeHelped maintain a focused learning environmentTracked students' progress in their personal learning plans

Master'S Degree, Bioengineering And Biomedical Engineering

Bachelor Of Biomedical Engineering, Magne Cum Laude

Activities and Societies: Biomedical Engineering Society, Chinese American Student Association, Society of Asian Scientists and Engineers

High School Diploma, 4.0

Activities and Societies: Speech Team, National Honor Society, Future Problem Solving