Meredith Wolfrom Email and Phone Number

Senior level associate in Molecular cloning group-review and assign clone requests, aid requesters with vector selection, verification of final sequence for all plasmids generated and testing new technologies.Experienced with Gateway, Infusion, ligation independent cloning (LIC) and traditional cloning techniques; shRNA cloning. Led high-throughput cloning initiative to create library for automated protein expression and purification system. Extensive experience with De novo cloning and sequence analysis of genes from orthologue species, including use of RACE PCR. Experienced with DNA sequencing included data analysis using Sequencer. Responsible for DNA Analyzers including ABI 3730xl and 3500xl, including routine maintenance, monitoring sequence quality and working with service engineers to resolve any instrument problems.Experience with Qiagen Universal BioRobot and Beckman-Coulter Biomek-FX and Biomek-NX. Utilized Nautilus LIMS, Clone Repository database and helped to develop and test new clone request and project tracking system. Experience with Gene Construction Kit, VectorNTI, Lasergene Software including EditSeq and Megalign, and BLAST programs for analysis of genes and vectors. Responsible for maintenance of cloning vector repository as well as vector construction and validation. Transient transfections into mammalian cell lines, Lentivirus production, Luciferase Assays, Taqman Assays. Presented at project team meetings and section meetings. Member of Medical Emergency Response Team.

High-Throughput Sequencing, DNA purification and sequencing-reaction set-up for ABI 3700 sequencers. Utilized automation systems including Hydra 384, Quadra Tomtec, Tecan, Velocity 11 BioCel, and Vprep 384 pipettor. Applied troubleshooting skills to resolve sample and equipment problems. SNP Analysis.