Michael Espiritu Email & Phone Number

Hillsboro, Or, Us

Portland, Oregon, United States

Hillsboro, Oregon

My research projects include studying enzyme kinetics and drug interactions of the breast cancer medication letrozole on Cytochrome P450s (CYP450) and studying the mechanism by which trans cinnamic aldehyde inhibits CYP2A6. Thus far, this opportunity has provided me with experience in in vitro drug metabolism, protein expression and purification, enzyme kinetics, and quantitative mass spectrometry using LC-MS/MS. I have gained experience with molecular modeling (Autodock), curve fitting with… Show more My research projects include studying enzyme kinetics and drug interactions of the breast cancer medication letrozole on Cytochrome P450s (CYP450) and studying the mechanism by which trans cinnamic aldehyde inhibits CYP2A6. Thus far, this opportunity has provided me with experience in in vitro drug metabolism, protein expression and purification, enzyme kinetics, and quantitative mass spectrometry using LC-MS/MS. I have gained experience with molecular modeling (Autodock), curve fitting with GraphPad Prism, Analyst, Multiquant, and Sciex triple quadrupole LC-MS/MS instrumentation. Show less

Hawaiian Islands

Recitation section of the course Biochemistry- BIO/MBBE-402 in the fall and Biochemistry lab 402L in the spring.These are introductory biochemistry courses that cover the basics of biochemistry and introduce students to vital biochemical pathways and principles.

Hawaiian Islands

My duties include the isolation of native peptide toxins through HPLC and their characterization through ESI-MS. My duties also include the investigation of novel synthetic peptide analogs synthesized through Solid Phase Peptide Synthesis (SPPS), folded synthetically through oxidative buffers such as ammonium bicarbonate, guanidine HCl, or rapid iodine, their purification through RP-HPLC, and confirmation through mass spectroscopy. This opportunity has provided me with experience in chemical… Show more My duties include the isolation of native peptide toxins through HPLC and their characterization through ESI-MS. My duties also include the investigation of novel synthetic peptide analogs synthesized through Solid Phase Peptide Synthesis (SPPS), folded synthetically through oxidative buffers such as ammonium bicarbonate, guanidine HCl, or rapid iodine, their purification through RP-HPLC, and confirmation through mass spectroscopy. This opportunity has provided me with experience in chemical synthesis, quantitative analysis through HPLC and MS, preparation of chemical samples through the use and maintenance of speed vac and freeze-dryer instrumentation, and experience with programs such as Empower, Millennium, and Analyst. Show less

Hawaiian Islands

My main duties included the preparation of samples, maintenance of equipment, interpretation of data, and preparation of reports. This opportunity has provided me with experience in liquid chromatography–mass spectrometry, nuclear magnetic resonance mass spectrometry, thin layer chromatography, Patch Clamp operation, and various associated programs such as Xcalibur, Agilent software, Microsoft Word, Patch Master, and Excel.

Doctor Of Philosophy (Ph.D.), Molecular Biosciences And Bioengineering

Bachelor Of Science (Bs), Biology

Activities and Societies: Chemistry club