Collaborated on the leading Virtual Power Plant application for distributed control of energy assets for a global customer base.

Identifying nuclear lamin protein interactions through coimmunoprecipitaton followed by liquid phase chromatography followed by mass spectrometry. Optimizing immunoprecipitations for clean protein complex purifications through chemically cross-linking an affinity purified antibody to a protein a/g bead.

Collected and analyzed DNA from patient blood samples for a variety of genetic disorders (Cystic Fibrosis, Fragile X Syndrome, Prader Wili & Angelman syndrome). Sequenced (Sanger & NGS) patient DNA and analyzed for mutations prevalent in cancer associated genes (c-KIT, NPM1, JAK2).

My primary responsibility was to generate K562 and MCF7 cells stably expressing bacterial artificial chromosomes (BACs) for the ENCyclopedia of DNA Elements (ENCODE) project. Each BAC was engineered to tag a single transcription factor with GFP. After the BAC has been transfected into K562 or MCF-7 cells, the expression of the fusion protein was validated via western blot and single cellular immunofluorescence staining. Upon passing both validations, the transcription factors were fixed to DNA for chromatin immunoprecipitation followed by sequencing (ChIP-seq). DNA sequencing libraries were then constructed and sequenced for the identification and cataloging of transcription factor binding sites for the known 1800 human transcription factors.