Mia Kumar Email & Phone Number

Albany, Ny, Us

Albany, Ny, Us

Albany, Ny, Us

-Marketed a vast portfolio of bioprocessing solutions to BioPharmaceutical and Biotechnology companies-History of exceeding target - 2020 (tracked 105%), 2019 (213%), 2018 (111%), 2017 (195%)-Experience managing academic, small-mid biopharma as well as large key accounts-Focused on strategic sales development, team leadership and account penetration in addition to quick wins -Consistently achieved multi-departmental sales of Sartorius’ Bioprocess portfolio at the same customer and at various customer accounts-Delivered targeted presentations to users, quality teams, managers, CEOs and VPs-Previous laboratory experience facilitates involvement with the customer at a technical level to better understand their needs and to identify the ideal solution for their process

Rockville, Maryland, Us

-Working in a BSL-2 and BSL-4 lab to grow and characterize viruses, including Cow Pox virus, Middle East Respiratory Syndrome virus, and Ebola virus. -Constructed Ebola virus phosphorylation site mutants (in a specific gene) and demonstrated the effect ofphosphorylation on viral transcription using the Ebola minigenome assay, analyzed by western blot, qPCR and flow cytometry.-Designed Ebola virus deletion mutants based on SHAPE data to understand the structure-function relationship of a specific region of RNA and the viral lifecycle, using the Ebola minigenome assay in a biosafety level 2 (BSL-2) lab, analyzed by qPCR and GFP expression in flow cytometry.-Demonstrated the efficacy of various fixatives in inactivating MERS and Ebola virus for the purpose of removing them safely from a BSL-4 containment lab to a lower BSL lab.-Designed and created ELISA plates for overseas MERS diagnostic assays, which led to the identification of camels as the host species.-Isolated and prepared primary cell lines for MERS infection to identify a potential MERS animal model-Performed numerous experiments using molecular virology and cellular biology techniques.

Rockville, Md, Us

-Assumed lead for new target cell line development projects.-Improved in-house capabilities for higher protein expression levels by leading efforts in vector design for 2 new products. -Utilized proficient bacterial transformation and cloning techniques for vector construction, including: PCR; ligation; bacterial culture; mini & maxi prep; and restriction digestion.-Performed transient and stable transfections of mammalian CHO cells using electroporation, producing multiple high titer isolates for each product.-Routinely perform batch culture, fed batch culture, transfection, single cell cloning and stability study experiments-Department representative for Continuous Improvement. Green belt certified-Acted as hiring manager for and supervisor of summer intern due to excellent communication and managerial skills.-Improved employee morale in the work place during the GSK acquisition, as a result of facilitative leadership training.-Served as Group lead for organizing the cell line freezer inventory, and implementing a new cataloging system for frozen samples, resulting in quick retrieval of stored sample location and information using LIMS software.

-Coordinated a research project for identifying predictive/novel biomarkers for diagnosis, prognosis and therapy of hepatocellular carcinoma (HCC) based on gene and microRNA (miRNA) array results.-Aided PI in design, direction, and initiation of new cholangiocarcinoma genomic profiling project using human clinical samples and mammalian cell lines from collaborating laboratories in Asia.-Independently executed experiments in a BSL-2 environment to analyze gene expression in HCC by assessing miRNA expression regulation in mammalian cell lines and human clinical samples.-Synthesized cDNA, and performed quantitative-PCR experiments (SYBR green and Taqman), in 96 and 384 well formats, of DNA and miRNA.-Performed protein analysis experiments, such as SDS-PAGE gel electrophoresis and Western blotting. -Demonstrated expertise in DNA and RNA isolation from cell and tissue samples.-Performed multiple sequence analysis and alignments using BLAST, DNA Star, and Clustal W programs.-Frequently presented progress reports for department.-Initiated and developed an intra-lab wiki-database for lab protocols, meetings, and communications which reduced the time spent searching the global database and Outlook.-Authored several peer-reviewed publications from research efforts.

-Identified novel biomarker for predicting early-stage ovarian cancer via siRNA knockdown assays in mammalian cell lines.-Bred, clipped tails and genotyped transgenic mice to study the Ras oncogene in cardiac hypertrophy.-Designed and conducted experiments to differentiate cell cycle stages during cardiac hypertrophy using immunohistochemistry, immunofloresence and Western blotting techniques probing various cytokines.-Produced and purified FLAG-tagged recombinant proteins using bacterial cultures.-Published peer-reviewed paper based on research efforts.-Established a database for tissue samples, easing identification of sample properties and storage location.-Trained 3 research fellows in various lab protocols such as Western blotting, DNA/RNA extraction and PCR.-Prepared buffers, ordered reagents, tracked routine calibrations of lab equipment and maintained safety in the lab to ensure a quality work environment.

-Managed a program for five Clinic Assistants providing health care services to Planned Parenthood clients. -Pivotal in disease prevention by educating patients about reproductive health and communicable disease. -Offered HIV counseling -Communicated with the local public health department. -Coordinated and trained the clinic staff to keep a timely schedule.

-Coordinated 5 supplemental classes for undergraduate science courses ranging from 5-20 students. -Developed weekly review sessions of lectures and problem solving. -Answered students' questions and offered constructive criticism during their experiments.

Master Of Science, Biotechnology, Concentration In Biodefense

Bachelor Of Science, Cellular And Molecular Biology