 Clonal relationship through molecular/DNA fingerprinting of pathogenic Vibrio cholerae from clinical and environmental samples (marine, fresh water, sea-foods and clinical samples). Molecular fingerprinting of lactic acid bacteria from food sources and marine environment. A biospropecting of molecular strains selections for improvements in the industrial line processes. Genetic analysis of virulence associated genes from Vibrio pathogenicity island-toxin co-regulated pili gene cascade using PCR-based probes in Vibrio cholerae non-O1 and non-O139. Genetic analysis of virulence associated genes from CTX genetic gene cascade using PCR-based probes in Vibrio cholerae non-O1 and non-O139. Physico-chemical and the microbiological quality of surface water from creek settlements in Bayelsa and Rivers States, South-south, Nigeria. Investigation of the environmental indices of cholera outbreaks in tropical climates