• Team lead for processing patient and healthy donor blood and tissue samples. • Isolation of cfDNA from processed donor plasma samples.• Characterization and QC of cfDNA for NGS library preparation for use on Illumina platforms. • Operation and maintenance of QiaSymphony liquid handler system.• Patient sample storage and database management.

• Transfection, harvest, and concentration of pseudotyped Lenti-virus.• Characterization of virus via western blotting, ELISA, qRT-PCR, and functional titers.• Isolated PBMCs from donor leukopaks and subsequent transduction for CAR-T cell production.• Maintenance of Jurkat, HeLa, Raji, and HEK293 cell lines for downstream CAR-T cell candidate screening.• Production of engineered cell lines constitutively expressing target surface proteins.• Generated numerous vectors for… Show more • Transfection, harvest, and concentration of pseudotyped Lenti-virus.• Characterization of virus via western blotting, ELISA, qRT-PCR, and functional titers.• Isolated PBMCs from donor leukopaks and subsequent transduction for CAR-T cell production.• Maintenance of Jurkat, HeLa, Raji, and HEK293 cell lines for downstream CAR-T cell candidate screening.• Production of engineered cell lines constitutively expressing target surface proteins.• Generated numerous vectors for ectopic expression of candidate targets through restriction enzyme and digestion independent based cloning techniques.• Small-to-large scale DNA isolation and quality control processing for companywide experiments and products Show less

• Designed, cloned, and QC’d mutated LAGLIDADG homing endonucleases in expression vectors. • Transformation of engineered protein vectors into expression and storage bacterial cell lines. • Expression and subsequent column-based purification of engineered proteins. • Characterization of candidate endonucleases via western blotting, time course and enzymatic efficacy assays. • Development and optimization of PCR and cloning strategies used for production. • Buffer and reagent… Show more • Designed, cloned, and QC’d mutated LAGLIDADG homing endonucleases in expression vectors. • Transformation of engineered protein vectors into expression and storage bacterial cell lines. • Expression and subsequent column-based purification of engineered proteins. • Characterization of candidate endonucleases via western blotting, time course and enzymatic efficacy assays. • Development and optimization of PCR and cloning strategies used for production. • Buffer and reagent preparation for lab wide experiments. Show less