Michael Caprio Email & Phone Number

Cambridge, MA

• Executed VH-VL In-Fusion HD molecular cloning of antibodies from hybridomas and primary B cells.
• Propagated and expanded engineered cell lines for immunizations into mouse models.

Cambridge, MA 02139

• Performed cell line development (CLD) through participation in transfection (electroporation) of plasmids, stable selection, sub-cloning, batch, fed-batch, and characterization of CHO suspension cell lines using the.
• Operated various equipment including Cytena Single Cell Printer, Cell Metric plate imager, Hamilton liquid dispenser, familiarity with OCTET, and small scale shaker flasks.

Cambridge, MA

• Performed p24 ELISA assays to determine amount of viral infection using SpectraMax plate reader.
• Implemented protocols for an ELISA benchtop workstation.

Cambridge, MA

• Received Randstad Reach Award for delivering CHO cells to support HTS screening efforts that were met before deadline.
• Maintained and provided CHO cells to support screening efforts that were met ahead of deadline.
• Performed HTS cell-based assay to determine if small molecule compounds were either cell cycle or non-cell cycle dependent.

Cambridge, MA

• Investigated acquired point mutation(s) resistance of Imatinib to CML cell line KCL-22 by performing RNA isolation, cDNA synthesis, primer design, PCR, and sequencing analysis techniques.
• Screened chromatin modifying agents to observe if drug resistance can be reversed in cancer cell lines.
• Executed in vitro AlphaLISA assay to screen compounds against H3K27me3 (EZH2) epigenetic target.
• Planned and executed the screening of over 1500 small molecule compounds across 10 cancer cell lines using a low-volume 96-well liquid handling workstation. Analyzed data using EXCEL XLfit and GraphPad curve-fitting.
• Developed SOP's for small volume pipetting to screen compounds using Caliper's Sciclone inL10 low-volume and Sciclone ALH 3000 liquid handling workstations.
• Established protocols for transfection and transduction of lenti-virus into suspension/adherent cell lines by determining parameters such as cell growth, stable cell lines via puromycin concentration, incubation times.