Michael Caprio Email & Phone Number

Cambridge, Ma

• Executed VH-VL In-Fusion HD molecular cloning of antibodies from hybridomas and primary B cells.• Propagated and expanded engineered cell lines for immunizations into mouse models.

Cambridge, Ma 02139

• Performed cell line development (CLD) through participation in transfection (electroporation) of plasmids, stable selection, sub-cloning, batch, fed-batch, and characterization of CHO suspension cell lines using the CHOZN format.• Operated various equipment including Cytena Single Cell Printer, Cell Metric plate imager, Hamilton liquid dispenser, familiarity with OCTET, and small scale shaker flasks.

Cambridge, Ma

• Performed p24 ELISA assays to determine amount of viral infection using SpectraMax plate reader.• Implemented protocols for an ELISA benchtop workstation.

Cambridge, Ma

• Received Randstad Reach Award for delivering CHO cells to support HTS screening efforts that were met before deadline.• Maintained and provided CHO cells to support screening efforts that were met ahead of deadline.• Performed HTS cell-based assay to determine if small molecule compounds were either cell cycle or non-cell cycle dependent.

Cambridge,Ma

• Investigated acquired point mutation(s) resistance of Imatinib to CML cell line KCL-22 by performing RNA isolation, cDNA synthesis, primer design, PCR, and sequencing analysis techniques. • Screened chromatin modifying agents to observe if drug resistance can be reversed in cancer cell lines.• Executed in vitro AlphaLISA assay to screen compounds against H3K27me3 (EZH2) epigenetic target.• Planned and executed the screening of over 1500 small molecule compounds across 10 cancer cell lines using a low-volume 96-well liquid handling workstation. Analyzed data using EXCEL XLfit and GraphPad curve-fitting and software to determine EC50 values.• Developed SOP's for small volume pipetting to screen compounds using Caliper's Sciclone inL10 low-volume and Sciclone ALH 3000 liquid handling workstations.• Established protocols for transfection and transduction of lenti-virus into suspension/adherent cell lines by determining parameters such as cell growth, stable cell lines via puromycin concentration, incubation times and virus addition.• Highly experienced in mammalian cell culture using aseptic techniques - propagation of adherent and suspension cell lines, mycoplasma testing, viability determination, cell banking and record management of cell lines.• Prepared, evaluated, and presented data internally to cross functional teams using PowerPoint software.