Michael Cicirelli, Phd Email & Phone Number

Seattle, Washington, United States

- Directed a small team of scientists to screen a 370K diversity library against ~50 orphan GPCR targets using a cell redistribution assay run on an automated robotics system with high content imaging instruments- Led the development and screening of several HTS assays against complement system serine proteases and lectin pathway assays- Wrote numerous VBA programs for automating data analysis and loading results into a Live Design database- set up a chemical repository and compound distributions system- wrote a program for integrating various instruments into a semi-automated compound weighing & dissolution workstation- developed a panel of enzymatic assays, along with members of the group for determining Ki values for complement system enzymes and directed the development and routine running of several lectin pathway assays to aid medicinal chemists with SAR- set up database tables for capturing screen data, chemical library and inventory data, assay performance and hits tracking tables

Seattle, Washington, United States

- Successfully set up a laboratory to print high density microarrays- Employed microarray technology for the HT screening of antibodies produced from activated B-cell clones.- Directed the capture of human antibodies on slide surfaces and developed assays for sensitive detection of specific antibodies, as well as total IgG- Directed screening against a variety of targets and wrote data analysis programs for quantitation of antigen binding and screen results reporting

Seattle, Washington, United States

Developed microarray applications for a high throughput microarray Surface Plasmon Resonance instrument, capable of measuring binding of proteins and small molecules to a maximum of 5000 arrayed spots simultaneously in real time. - Tested the capabilities of early prototype instruments and provided feedback on the fuluidics, optics and software design, as well as testing practical applications.- Led efforts to develop user requirements for the instrument, evaluated microarray printing technologies and optimized array printing- Directed efforts to identify and optimize new microarray applications and product ideas for proteomics research

Seattle, Washington, United States

Director, Research (2001-2006) - Directed a team of 6 highly experienced scientists to continue running HTS and develop additional capabilities, adding value to the company - Led a group effort to develop and run routine automated assays for solubility, chemical stability, metabolism, permeability (Caco2), serum protein binding, and hERG - wrote extensive VBA code for creating LC/MS/MS batch files, data acquisition, automated analysis and reporting - directed the set up of automated system for screening mAbs and small molecules against proliferating cell lines - led the development of a company-wide chemical repository and successful launch - managed the relocation of our screening and chemical repository to a new building and was involved in optimizing the new lab design and setting up the robotics and equipment

Bothell, Washington, United States

Managed a team of five talented technical scientists and RAs - performed HTS on a wide variety of biochemical and cell- based targets (~80 total) - procured and screened diverse synthetic libraries, combinatorial libraries, natural products, focused libraries, and peptidomimetics (~300,000 compounds)

Bothell, Washington, United States

Established and managed HTS program, trained RAs - Helped research groups develop "automation-friendly" and "kinetically-correct" assays for HTS - Devised and employed compound pooling strategy - procured and programmed robotic systems for formatting compounds and running HTS - Set up a storage facility for library compounds -wrote software for analyzing screen data and storing screen results

Bothell, Washington, United States

- Developed robust automation-friendly radiometric assay for PDEs - Refolded PDEs from inclusion bodies and characterized their enzyme kinetic properties - Became involved in strategic planning for a small molecule screening program

Gaithersburg, Maryland, United States

- R&D, transfer of technologies to operations, technical support, product planning, teaching, writing protocols, manuscripts and catalog copy- overexpressed protein kinases and PDEs in Ecoli and engineered them for facilitated protein purification- purified proteins and wrote up detailed protocols- developed assay kits for protein kinases and phosphatases- developed a highly sensitive assay for inorganic phosphate- identified, procured and characterized inhibitors of protein phosphatases and kinases for inclusion in the LTI product line

Post-Doc With Ed Krebs At The Howard Hughes Medical Institute, Protein Biochemistry

Doctor Of Philosophy - Phd, Developmental Biology (Mentor: Dr L.D. Smith)

Bachelor Of Science - Bs, Biology