Michael Spelman Email & Phone Number

Oakland, Ca, Us

Oakland, Ca, Us

Emeryville, California, Us

● Designed, tested, and optimized knock-in payloads compatible with proprietary gene-editing tools to enable the development of an autologous “Dual” CAR-T product with specific dual-target functionality relevant to the therapeutic target and the stable expression of a “safety-switch” moiety that enabled depletion of the engineered CAR-T cells.● Developed cell and molecular assays to evaluate the functional activities of CAR-T and safety-switchexpressing T cells, incorporated the use of various reagents and instrumentation for high-throughputreadouts, and generated complete data packages as part of IND-enabling POC studies.● Collaborated across departments to generate a multiplex-edited, hypo-immunogenic allogeneic CAR-T product, utilizing both nucleases and base editors in combination with viral and non-viral therapeutic payload delivery.

Emeryville, California, Us

● Identified and evaluated relevant genes of interest (GOIs) in terms of knock-out efficiency usingMetagenomi’s proprietary gene-editing toolkit as well as assessed nominated GOIs based on targetedinsertion efficiency using a therapeutic payload in the context of an allogeneic CAR-T product.● Collaborated with multiple teams to refine the list of relevant GOIs through the identification ofbiological and technical roadblocks, evaluate novel nuclease activity at these GOIs, and quantitativelyassess knock-out and knock-in efficiency using indel analysis (NGS), and expression of endogenous and exogenous proteins at the cell surface via flow cytometry, immunofluorescence, and cytokineproduction.● Designed, tested, and optimized knock-in payloads and their ability to specifically redirect functionalactivity of the engineered T cells using CARs, as well as support the overall health and fitness of the cells by way of secreted or tethered cytokines.

Brisbane, California, Us

● Planned and implemented experiments to advance project and department therapeutic targets and aid in effective decision-making in pursuit of the Cell Therapy group and company-wide goals.● Designed and screened DNA plasmids for use in transfections, site-specific targeted integration,lentiviral production and transduction, and control/target/reporter cell line engineering.● Developed and characterized CAR expression vectors for the engineering of autologous and allogeneic cell products for application across various oncological, auto-inflammatory, and infectiousdisease indications.● Collaborated across research groups, company sites, and partner organizations to explore and implement new methodologies, optimize workflows, and identify therapeutic applications and product concepts.

Brisbane, California, Us

● Developed, optimized, and implemented new flow cytometry panels (basic phenotyping, characterization of differentiation state, activation/exhaustion, knock-out/knock-in of target genes, etc.), reagents, workflows, and instrumentation within the Immuno-Oncology research group, up to 11 colors. ● Provided training and support to promote a greater understanding of the instrumentation, capabilities of flow analysis, and facilitate ongoing lab activities of members of various research groups. ● Designed, tested and optimized multiple assays, including NK cell functional assays, cell cytotoxicity and immune evasion assays, primary cell characterization, flow data collection and analysis, cell modification experiments, cell-line engineering, and cell outgrowth and banking for future studies.● Developed and functionally evaluated new technologies associated with allogeneic cell therapies and immune system evasion.

● Worked within the Molecular Engineering group to generate expression vectors for transgenes thatencoded tunable, drug-inducible CAR-T proteins for more robust immunological responses in patientswith diseases such as Acute Myeloid Leukemia (AML).● Explored multiple strategies to improve the vector construction process to support research requirements to support our partnership with Kite Pharma, as well as the needs of the broader organization.

● Established and operated initial tech transfer experiments to adapt the CLARITY technique for use with human tissues. ● Investigated and applied the use of numerous antibodies, protein assays, and imaging techniques to assess the extent and quality of tissue clarification and compare various tissue clearing conditions.

San Luis Obispo, Ca, Us

San Diego, California, Us

● Explored the use of media supplements, such as TGF-b and IFN-y, to influence endogenous structural protein synthesis by normal human lung fibroblasts (NHLF) and bronchial smooth muscle cells (bSMCs) within 3-D bioprinted small airway tissue models. ● Assessed the cellular and extracellular matrix compositions of treated airway models by way of IHC, IF, and physical strength testing following mechanical conditioning on a perfusion/flow bioreactor.

Santa Barbara, Ca, Us

Worked to increase automotive safety using various cameras, hardware, and our software program.Facilitated the management of over 100 clients and their fleets of commercially used vehicles such as taxi cabs, ambulances, armored cars, coach buses, and security vehicles.Repaired the cameras, installed and maintained software remotely on clients’ computers, assisted in customer satisfaction and customer relations, as well as contributed to sales and apprehension of new clientele.

Master'S Degree, Biological Sciences With A Specialization In Stem Cell Research

Bachelor Of Science (Bs) And Bachelor Of Arts (Ba), Biopsychology And Biology