Michael Lange Email and Phone Number

Peptide design and assay development for a biased GPCR agonist.

-Building an RNA sequencing pipeline to integrate with current process characterization efforts-Scaling down routine process characterization studies using ambr250 bioreactors-Simplifying and standardizing statistical calculations through automation in JMP

-Characterizing novel modalities of clinical interest in Regeneron's pipeline through Design of Experiments-Building and automating a model confirmation program for evaluation of small scale models using manufacturing data (lifecycle management of lab-scale models over time)-Implementing automation technologies for increased bioreactor monitoring and decreased time spent on manufacturing floor-Combining RNA-seq data with Design of Experiments techniques to deepen process understanding (link cell biology with engineering knowledge)-Collaborate cross-functionally to improve study efficiency through the development of scale-down models in Ambr250-Pioneering use of JMP statistical software to facilitate our Design of Experiments analysis

-Designed and executed a custom DOE to investigate endotoxin deactivation during viral inactivation in a cGMP environment-Proved that base adjustment is a critical factor in endotoxin deactivation for one of Regeneron’s molecules-Cultivated a wide understanding of process qualification and validation, manufacturing, data monitoring CMC, drug development and unit ops such as TFF, UFDF, Filtration, ProA, AEX/CEX, and HIC chromatography

I tutored grades 9-12 in various mathematics classes, physics, biology and biotechnology, and chemistry. I always developed problem solving methodologies with students so that they can continue to make progress outside of tutoring. I enjoy working one on one with the students and developing relationships with them, so that they can feel comfortable consistently seeking help from me.

My work focused on using ammonium-functionalized carbon nanotubes as a nucleic acid delivery platform to the kidneys. - Used small interfering RNA as a therapy to prevent acute kidney injury - Revealed a pro-inflammatory gene response that was unaffected by the drug being tested - Proposed novel targets based on the gene responses for future studies with carbon nanotubesAdditionally, I also studied the uptake of carbon nanotubes by peritoneal macrophages. This was a proof of concept study for immunotherapy research targeted towards macrophages in the tumor microenvironment.

I worked with four other teaching assistants (TA's) to develop a synthetic biology program for high school students: - Taught students common laboratory practices and how to design cloning experiments - Guided student group-work to develop a biosensor using iGEM BioBrick parts and information from scientific literature - Taught students how to communicate what they built and learned to a general audience - Organized meetings with the other TA's to plan teamwork-building activities, like constructing DNA ladders out of paper.

I worked under a graduate student, where I learned several molecular biology skills and proper experimental design. Some of my tasks included: - Investigating the sequestration of rhinovirus RNA in upper airway epithelial cells through capsid swap experiments - Redesigning primers for optimization and troubleshooting of the Gibson Assembly on recombinant rhinovirus vectors - Performing digestion and ligation reactions and PCR, preparing agarose gels, purifying and precipitating DNA samples - Analyzing purity of recombinant DNA via spectrophotometry