Michael Peracchio, Ms, Cgc, Mb(Ascp) Email & Phone Number

Remote (Headquarters In Stamford, Ct)

Branford, Connecticut, United States

Farmington, Ct

Hamden, Ct

Courses taught/teaching: BIO 101L – General Biology I Lab and BIO 102L – General Biology II Lab• Introduce and lead laboratory experiments for undergraduate students in health sciences majors• Create and grade assignments, quizzes, and exams, and manage any student issues that may arise• Laboratory exercises develop skills in experimental design, data analysis and scientific writing• BIO 101L content emphasis is on basic concepts of life science with emphasis on the methods of science, the chemistry of life, and molecular and cellular evolution• BIO 102L content emphasis is on animal anatomy and physiology, animal reproduction and development, the nervous system, evolutionary mechanisms and ecological principles.

Branford, Ct

Sema4 is an interdisciplinary health information company that is committed to providing open access to data and creating practical tools that help patients, clinicians, and researchers better predict health trajectories.

Mount Sinai Genetic Testing Laboratory - Branford, Ct

 Perform DNA and RNA extraction from various sample types (blood, saliva, tissue) for clinical and research samples using the QIAsymphony Perform quality control procedures (quantification, sample quality assessment) using VolumeCheck, Trinean DropSense96, and Qubit 3.0 fluorometer on DNA and RNA from clinical and research samples Developed protocol for Sanger sequencing sample preparation for clinical confirmatory testing Perform sample normalization and AmpliSeq automated library preparation using Hamilton STAR for sequencing on Ion Torrent Proton or S5 XL next-generation sequencers Use laboratory information management system (LIMS) to track samples throughout processing workflow Follow all appropriate laboratory practices to maintain compliance in CLIA-certified environment Perform routine daily, weekly, and monthly cleaning and preventative maintenance on laboratory equipment

Genetics And Genomics

• Characterization of novel small RNAs produced by the Epstein-Barr virus (EBV) and investigation of their roles in EBV-associated cancers• DARPA-funded large-scale human genomics/transcriptomics analysis• Mining GRO-seq data for evidence of transcription start sites and active promoters within human centromeric alpha-satellite sequencesSKILLS & ABILITIES • Illumina library preparation and sequencing for human RNA-seq on MiSeq and NextSeq platforms• Human transcriptome microarray sequencing and analysis using Affymetrix Gene Atlas System• Primary and secondary bioinformatic analysis of human genomic and transcriptomic NGS data using various Unix command line and online programs• Clinical genomic data analysis with NCBI, UCSC, OMIM, Cancer Genome Anatomy Project (CGAP), Mitelman Database, Atlas of Genetics and Cytogenetics in Cancer• Preparation of example clinical reports based on genomic data analysis• Cell culture of mammalian (human/primate) adherent and suspension cell lines• Chromosome harvest, slide preparation, G-banding, immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH), including RNA FISH, and karyotyping (utilizing ISCN nomenclature)• Imaging analysis of normal and transformed human cells using fluorescent microscopes• Total and small RNA isolation, DNA isolation, in vitro transcription (IVT), and quality assessment• PCR/RT-PCR, real-time qPCR/qRT-PCR, gel electrophoresis, Northern and Western blotting• Sanger sequencing, including primer design, gene isolation, molecular cloning, and data analysis• Transfection of customized vectors by electroporation and chemical methodologies• Trained and mentored two undergraduate students working on various aspects of EBV project• Laboratory management responsibilities including organization, inventory, and ordering of lab samples, supplies, and equipment

Genetics And Genomics

The Genetics Revolution in Contemporary Culture – Fall 2013, 2014, 2015• Organized and directed discussions about current topics in genetic engineering and biotechnology with six sections of 16 freshman and sophomore honor students• Managed students within classroom and resolved issues that arose outside of the classroom• Scheduled and conducted exam review sessions, graded course exams, and guided and evaluated student group projects and presentationsGenetic Engineering and Functional Genomics – Spring 2014• Organized, scheduled, and directed honors section discussions of popular films related to the content of the course with 25 undergraduate studentsPrinciples of Biology I – Fall 2012, Spring 2013• Taught introductory laboratory biology, including basic molecular biology techniques, to over 150 students; managed student issues in and out of class, and graded lab reports, presentations, and lab practical exams

Hartford, Ct

• Worked with 12 high school students and CSC staff members to help students create mini-exhibits focused on genetics and genomics based on previous survey results addressing knowledge gaps of the general public• Monitored student implementation of exhibits on museum floor and provided critical assessment and formative evaluation of public engagement for improvement of exhibits

• Taught various science subjects including Biology (AP, Honors, College Prep, and General levels) and Energy & Matter (Honors and College Prep levels) to students in grades 9-12• Designed and implemented lessons for groups of students of varying abilities and learning styles• Demonstrated effective classroom management to create a safe and positive learning environment• Effectively communicated with parents about student progress, both positive and negative• Collaborated with other faculty, administration, and staff in faculty and department meetings, pupil placement team (PPT) meetings, and professional development activities• Served as faculty adviser to the National Honor Society and the student led Earth Club • Coached Varsity Boys Basketball (2 years) and JV Boys Basketball (3 years)

Master'S Degree, Genetic Counseling

Master’S Degree, Molecular And Cell Biology - Genetics And Genomics

Master’S Degree, Secondary Education - Science

Bachelor’S Degree, Ecology And Evolutionary Biology, Magna Cum Laude

Activities and Societies: Minor in Molecular and Cell Biology; Golden Key National Honor Society; New England Scholar – 2003