Michael Duffield Email & Phone Number

Durham, North Carolina, US

Glen Allen, VA, US

• Worked independently and as part of an interdisciplinary team to evaluate non-viral gene therapies using patented 3-DNA scaffold technology.
• Developed and optimized cell-based assays to assess potency and durability of genetic payloads, using microscopy, ddPCR and other molecularbiology techniques.
• Designed and generated technical protocols and SOPs in accordance with GLPs and tracked samples using a Laboratory InformationManagement System.

US

• Perform hands-on-analysis of brain tissue obtained from rodent models of CNS diseases.
• Develop, optimize, and conduct biochemical assays with brain tissue or cultured primary and immortalized cell lines.
• Design and optimize a cellular excitotoxicity assay to investigate novel neuroprotective treatments.
• Optimize neuronal differentiation protocols to generate hippocampal neurons to explore underlying pathology in AD and other neurodegenerative diseases.

Atlanta, Georgia, US

• Design and execute cellular experiments and screens using shRNA, CRISPR/Cas9, and other functional genomics tools for target identification, MOA characterization, and mechanisms of resistance and/or sensitivity to.
• Regularly perform cell culture in viral BSL-2 lab for small scale assay optimization and large screens, as well as qPCR, gDNA extractions, and additional molecular biology techniques.
• Maintain an organized and detailed electronic laboratory notebook (Signals, Biovia).

Tarrytown, New York, US

-Investigating the role of microglia in neurodegeneration using human iPSC-derived microglia on multiple assays, including phagocytosis, chemotactic migration, and viability assays.-Explore efficacy and mechanisms of action of siRNA and antisense oligonucleotides in vitro and in vivo.-Optimize and implement neuronal differentiation protocols in embryonic.

New York, NY, US

• Investigated the underlying mechanisms of neurodegeneration using human iPSC-derived neurons containing mutations that cause frontotemporal dementia and other neurodegenerative diseases.
• Designed and generated stable mutant APP/PSEN cell lines through CRISPR/Cas9 genome editing in induced pluripotent stem cells.
• Facilitated neuronal differentiation of iPSC lines through Dual SMAD inhibition and cultivated cortical neurons for 3D co-culture with astrocytes and other microglial cells for further analyses.
• Assisted in managing laboratory operations during a relocation period, including rodent colony maintenance, and lab inventory monitoring and purchasing.

Philadelphia, Pennsylvania, US

• Identified a key residue on glutamate transporter EAAT2 at the interface of the scaffolding and transportation Domains that when mutated, resulted in a 300% uptake of extracellular glutamate, and hold potential for.
• Designed and investigated the effects of additional local point mutations to further explore the mechanism of action of the increased glutamate uptake.
• Compared EAAT2 surface protein expression between mutant and wild-type transporters through BS3-crosslinking and surface biotinylation.
• Identified several novel compounds derived from spider venom components which provide neuroprotection when bound to the EAAT2 activation domain through hybrid structure-based virtual screening.
• Explored any off-target effects of novel compounds on other neurotransmitter transporters, including serotonin, dopamine, and norepinephrine in vitro.

I recorded patient vital signs including: blood pressure, heart rate, body temperature, and height/weight. I also provided the physician a detailed medical history for new patients. Additionally, I was tasked with administering injections and immunizations, as well as Electrocardiograms.

Jenkintown, PA, US

I was tasked with assisting students in understanding course material in a number of courses (Chemistry, Biology, Math, and Anatomy & Physiology). I reviewed homework assignments and returned tests. I provided effective study strategies and review methods for academic success.

Collegeville, PA, US

• Investigated the correlation between body perception and eating attitudes in female undergraduates.
• Organized and analyzed data as head of data management group.

Collegeville, PA, US

• Examined the effects of several depressants on the medulla using a weakly electric fish bio-assay.
• Conducted experiments and interpreted data.
• Presented results at The 2011 Ursinus College Summer Fellows Research Symposium, 2011 Mid-Atlantic Pharmacological Society at Thomas Jefferson University, and The 2012 Ursinus College Celebration of Student Achievement.

Collegeville, PA, US

• Researched automaticity in grapheme-color synesthesia by studying brain activity using electroencephalography.
• Prepared subjects for test administration and attached the EEG apparatus.

Collegeville, PA, US

• Managed two sections of Biology101 Lab.
• Graded all assignments and kept gradebook up to date.
• Aided students in performing experiments and understanding results.

Brisbane, QLD, AU

• Processed customer orders
• Prepared pizzas and readied them for delivery
• Kept the store clean and organized
• Delivered orders if necessary

• Scheduled appointments and events.
• Authorized electronic referrals and called in prescriptions.
• Filed physician reports and organized patient charts.

Master Of Science (Ms), Drug Discovery And Development

Bachelor'S Degree, Neuroscience & Psychology