Michael Wilhide, Ms, Mb(Ascp)Cm Email & Phone Number

Cincinnati, Oh, Us

Edison, Nj, Us

Contractor at P&G Global Biotechnology Department- Beauty Skin Care• Visualized skin compartments by staining prepped histological slides for Laser Capture Microdissection (LCM).• Accurately isolated stratum basale layer, super basale layers, sebaceous glands, hair roots and dermis from over 200 clinical human skin biopsies using Zeiss PALM MicroBeam LCM system. A colleague and I completed the project two months ahead of schedule• Prepared the collected fractions for RNA isolation, quality control, and RNA sequencing analysis.

Cincinnati, Oh, Us

Lab Management• Managed a high-profile cardiometabolic biology research laboratory.• Interviewed, trained and supervised a team of student employees. • Monitored and optimized monthly supply budget.• Coordinated the procurement process, placing orders, and maintaining cost-effective supply inventory.• Implemented and oversaw stringent lab safety protocols, ensuring compliance with university policies and federal regulations.Collaboration and Support• Provided support and assistance to fellow lab members, promoting a positive and collaborative work culture. Enhanced the overall efficiency of the research team.Mouse Colony Management• Organized and maintained a large, intricate mouse colony. • Conducted genotyping procedures for multiple mouse lines, contributing to the advancement of laboratory research goals.

Seattle, Wa, Us

High Complexity Diagnostic Molecular Test in a CLIA, CAP, and ISO 15189 Accredited LaboratoryAccurate Test Results Delivery• Consistently delivered precise test results within stipulated service-level agreements, ensuring a customer-driven approach in a high-throughput diagnostic molecular laboratory.High-Throughput Lab Operations• Played a crucial role in processing and testing over two million samples, including comprehensive testing for SARS-CoV-2.Team Coordination and Training• Functioned as a team coordinator and member, overseeing the processing of patient samples according to Good Laboratory Practice (GLP) and Standard Operating Procedures (SOPs).• As a Trainer Ambassador, facilitated training sessions for personnel on automated liquid handling, high-throughput automated equipment, SOPs methods and procedures, and troubleshooting protocols.Equipment Maintenance and Compliance• Ensured compliance of equipment and instruments within the SOPs and accreditation standards, promptly recognizing and addressing malfunctions, and troubleshooting common errors.• Documented and escalated protocol deviations, providing valuable suggestions for improvements in the laboratory information management system (LIMS).Quality Assurance and Professional Development• Practiced Good Document Practice (GDP) and meticulous diligence, guaranteeing accurate test results and compliance with regulatory agency requirements.• Maintained an understanding of current SOPs and completed CAP Continuing Education courses, including ISO 15189 2021, Quality Culture, Root Cause Analysis, Internal Auditing, Management Review, Mistake Proofing, QMS Implementation Roadmap, Document Control, and Quality Manual Development.

High Complexity Molecular Diagnostic Blood Test in a CLIA Certified LaboratoryRNA Isolation Expertise• Proficiently isolated total RNA from stroke patient blood using the PAXgene blood system and QIA cube. Quality control to ensure the integrity and purity of genetic material.Affymetrix Microarray Platform Execution• Executed Affymetrix microarray platform to identify the type of cerebrovascular events in clinical samples, leading to more precise diagnosis and clinical treatment. SOP Creation and Enhancement• Played a pivotal role in the creation and improvement of Standard Operating Procedures (SOPs). • Assisted in basic administrative tasks related to clinical trial data and sample management. Diagnostic Lab Management• Shared responsibilities for the maintenance, operation, and quality control duties in the clinical diagnostic lab, ensuring the highest standards of performance.• Trained new clinical technicians on equipment usage, standard procedures, and lab maintenance protocols.

Us

Placenta-Specific Gene Therapy Enhancement• Enhanced the selectivity of placenta-specific expression of growth factor DNA for gene therapy. Molecular Cloning and Specificity Determination• Determined microRNAs with the potential to express the growth factor DNA, specifically in the placenta.• Conducted molecular cloning of miRNA target sites to the growth factor DNA sequence. Transfection Studies• Transfected placenta choriocarcinoma cells (BeWo cells) with the newly isolated and purified growth factor DNA containing microRNA target sites.• Measured gene expression to validate the effectiveness of the placenta-specific gene therapy approach.Lab Operations and Safety Compliance• Managed lab operations, including ordering necessary supplies and ensuring compliance with safety protocols.• Administered a safe and efficient working environment in the laboratory.

Cincinnati, Oh, Us

Pharmaceutical and FDA Regulated Environment• Operated in a Pharmaceutical and FDA regulated environment at Lilly Research Laboratories. Proficient Project Execution• Delivered proficient and prompt services to successfully complete internal client projects, meeting stringent quality requirements.Mammalian Cell Culture Expertise• Maintained and troubleshot a variety of mammalian, cancer, and natural killer cell culture lines. Cell Processing for Experimental Studies• Prepared and processed cells for experimental studies and high-throughput screening, contributing to the successful execution of research initiatives.Stable Cell Line Generation• Generated stable cell lines using the lentiviral expression systems. Electronic Documentation and Notebooking• Recorded laboratory documentations and procedures through electronic notebooking, ensuring accuracy and accessibility of essential data.

Columbus, Oh, Us

Research Focus: Immunotoxicity in Induced Traumatic Brain Injury (TBI) Rat Model• Established the principal investigator’s laboratory, overseeing equipment and supplies determination and ordering. • Managed workplace safety, good laboratory practices (GLP), and compliance with hospital policies and federal regulations.• Contributed significantly to the development of a rat traumatic brain injury (TBI) model.• Conducted measurements of cytokines and S100b levels in lipopolysaccharides (LPS)-stimulated blood cultures of sham and rat TBI models.• Implemented behavioral tests on rats, including rotarod, Barnes maze, and open field tests.• Optimized neurological stains, including Cresyl Violet/Nissl Stain, Luxol Fast Blue, Hematoxylin & Eosin.• Demonstrated proficiency in a range of molecular biology techniques, including ELISA and tissue processing.

Columbus, Oh, Us

Lab Management• Managed start-up laboratory, handling equipment and supplies determination and ordering. • Interviewed, trained, and supervised new laboratory personnel, along with co-mentoring a medical student. • Ensured highest standard in laboratory safety and compliance with hospital research policies and federal regulations.• Organized and managed laboratory mouse colony, human and mouse urine sample collections. Research Goal: Identification of Early Biomarkers for Congenital Obstructive Nephropathy (CON)• Processed both human and mouse urine for RNA isolation to accomplish quantitative PCR (qPCR), microRNA, and gene expression assays.• Performed small animal ultrasound of kidneys and bladders, isolated and micro-dissected embryonic kidneys, and bladders. Collected tissues and performed high-quality RNA isolation for qPCR, microarrays, and RNA sequence analysis. • Analyzed and executed PCR, qPCR, immunohistochemistry (IHC) immunofluorescence (IF), and in situ hybridization (ISH) for microRNA quantification and localization on tissue selections. Key Achievements• Successfully performed survival surgeries, including unilateral ureteric obstruction (UUO) and cutaneous vesicostomy.• Collaborated with other labs, leading to published papers.• Discovered that miR-205 is increased with the severity of congenital obstructive nephropathy (CON) in a mouse model.• Presented research in regional and national research conferences. • Attended 2015 Vanderbilt University Mouse Kidney Injury Workshop with direct training in UUO, cecal ligation and perforation, ischemia/reperfusion (dorsal IR and ventral IR) and 5/6 nephrectomy.

Cincinnati, Ohio, Us

Research Focus: Nuclear Factor Kappa-B (NF-kB) in Myocardial Ischemic Insults• Designed in vivo and in vitro experiments to investigate NF-kB's role in myocardial ischemic insults, confirming its activation and determining the timing of NF-kB-dependent cardioprotection. • Employed several molecular biology techniques, for example DNA, RNA, and protein isolation from tissues, ELISA, EMSA, IHC, and Western Blots to unravel the mechanism behind NF-kB-dependent cardioprotection following ischemic preconditioning (IPC) and permanent coronary artery occlusion (PO), as well as NF-kB-dependent cell death after ischemia/reperfusion (I/R).• Validated gene expression results from microarray and qPCR analysis, establishing novel genes involved in NF-kB-dependent outcomes.• Employed knockout mouse models based on scientific literature to examine the mechanism of specific genes (e.g., hspa1a and hspa1b). Research Outcome• Identified that NF-kB regulates 16 genes in NF-kB-dependent cardioprotection after Permanent Occlusion (PO) and 59 genes in NF-kB-dependent cell death after Ischemia/Reperfusion (I/R).• Discovered that Heat Shock Protein 70.1 (hspa1b) underlies NF-kB-dependent cardioprotection after PO and NF-kB-dependent cell injurious effects after I/R.• Identified therapeutic targets suitable for clinical development in treating ischemic heart disease.• Supported lab surgeons with in vivo myocardial ischemic models.Research Dissemination• Presented research results in published manuscripts, posters at national research conferences, local seminars, and laboratory meetings.

Cell Culture Maintenance• Successfully maintained and prepared various cancer cell cultures, contributing to the ongoing research in oncology.Proliferation and Apoptotic Cell Death Analysis• Conducted analysis on human tumor paraffin sections, specifically examining proliferation using PCNA Staining and apoptotic cell death through TUNEL assays.

Ms, Molecular, Cellular, And Biochemical Pharmacology

Bs, Biology (Concentration In Molecular Biology)