Michael Wilhide, Ms, Mb(Ascp)Cm Email & Phone Number

Cincinnati, OH, US

Edison, NJ, US

• Contractor at P&G Global Biotechnology Department- Beauty Skin Care
• Visualized skin compartments by staining prepped histological slides for Laser Capture Microdissection (LCM).
• Accurately isolated stratum basale layer, super basale layers, sebaceous glands, hair roots and dermis from over 200 clinical human skin biopsies using Zeiss PALM MicroBeam LCM system. A colleague and I completed the.
• Prepared the collected fractions for RNA isolation, quality control, and RNA sequencing analysis.

Cincinnati, OH, US

• Lab Management
• Managed a high-profile cardiometabolic biology research laboratory.
• Interviewed, trained and supervised a team of student employees.
• Monitored and optimized monthly supply budget.
• Coordinated the procurement process, placing orders, and maintaining cost-effective supply inventory.
• Implemented and oversaw stringent lab safety protocols, ensuring compliance with university policies and federal regulations.Collaboration and Support

Seattle, WA, US

• High Complexity Diagnostic Molecular Test in a CLIA, CAP, and ISO 15189 Accredited LaboratoryAccurate Test Results Delivery
• Consistently delivered precise test results within stipulated service-level agreements, ensuring a customer-driven approach in a high-throughput diagnostic molecular laboratory.High-Throughput Lab Operations
• Played a crucial role in processing and testing over two million samples, including comprehensive testing for SARS-CoV-2.Team Coordination and Training
• Functioned as a team coordinator and member, overseeing the processing of patient samples according to Good Laboratory Practice (GLP) and Standard Operating Procedures (SOPs).
• As a Trainer Ambassador, facilitated training sessions for personnel on automated liquid handling, high-throughput automated equipment, SOPs methods and procedures, and troubleshooting protocols.Equipment Maintenance.
• Ensured compliance of equipment and instruments within the SOPs and accreditation standards, promptly recognizing and addressing malfunctions, and troubleshooting common errors.

• High Complexity Molecular Diagnostic Blood Test in a CLIA Certified LaboratoryRNA Isolation Expertise
• Proficiently isolated total RNA from stroke patient blood using the PAXgene blood system and QIA cube. Quality control to ensure the integrity and purity of genetic material.Affymetrix Microarray Platform Execution
• Executed Affymetrix microarray platform to identify the type of cerebrovascular events in clinical samples, leading to more precise diagnosis and clinical treatment. SOP Creation and Enhancement
• Played a pivotal role in the creation and improvement of Standard Operating Procedures (SOPs).
• Assisted in basic administrative tasks related to clinical trial data and sample management. Diagnostic Lab Management
• Shared responsibilities for the maintenance, operation, and quality control duties in the clinical diagnostic lab, ensuring the highest standards of performance.

US

• Placenta-Specific Gene Therapy Enhancement
• Enhanced the selectivity of placenta-specific expression of growth factor DNA for gene therapy. Molecular Cloning and Specificity Determination
• Determined microRNAs with the potential to express the growth factor DNA, specifically in the placenta.
• Conducted molecular cloning of miRNA target sites to the growth factor DNA sequence. Transfection Studies
• Transfected placenta choriocarcinoma cells (BeWo cells) with the newly isolated and purified growth factor DNA containing microRNA target sites.
• Measured gene expression to validate the effectiveness of the placenta-specific gene therapy approach.Lab Operations and Safety Compliance

Cincinnati, OH, US

• Pharmaceutical and FDA Regulated Environment
• Operated in a Pharmaceutical and FDA regulated environment at Lilly Research Laboratories. Proficient Project Execution
• Delivered proficient and prompt services to successfully complete internal client projects, meeting stringent quality requirements.Mammalian Cell Culture Expertise
• Maintained and troubleshot a variety of mammalian, cancer, and natural killer cell culture lines. Cell Processing for Experimental Studies
• Prepared and processed cells for experimental studies and high-throughput screening, contributing to the successful execution of research initiatives.Stable Cell Line Generation
• Generated stable cell lines using the lentiviral expression systems. Electronic Documentation and Notebooking

Columbus, OH, US

• Research Focus: Immunotoxicity in Induced Traumatic Brain Injury (TBI) Rat Model
• Established the principal investigator’s laboratory, overseeing equipment and supplies determination and ordering.
• Managed workplace safety, good laboratory practices (GLP), and compliance with hospital policies and federal regulations.
• Contributed significantly to the development of a rat traumatic brain injury (TBI) model.
• Conducted measurements of cytokines and S100b levels in lipopolysaccharides (LPS)-stimulated blood cultures of sham and rat TBI models.
• Implemented behavioral tests on rats, including rotarod, Barnes maze, and open field tests.

Columbus, OH, US

• Lab Management
• Managed start-up laboratory, handling equipment and supplies determination and ordering.
• Interviewed, trained, and supervised new laboratory personnel, along with co-mentoring a medical student.
• Ensured highest standard in laboratory safety and compliance with hospital research policies and federal regulations.
• Organized and managed laboratory mouse colony, human and mouse urine sample collections. Research Goal: Identification of Early Biomarkers for Congenital Obstructive Nephropathy (CON)
• Processed both human and mouse urine for RNA isolation to accomplish quantitative PCR (qPCR), microRNA, and gene expression assays.

Cincinnati, Ohio, US

• Research Focus: Nuclear Factor Kappa-B (NF-kB) in Myocardial Ischemic Insults
• Designed in vivo and in vitro experiments to investigate NF-kB's role in myocardial ischemic insults, confirming its activation and determining the timing of NF-kB-dependent cardioprotection.
• Employed several molecular biology techniques, for example DNA, RNA, and protein isolation from tissues, ELISA, EMSA, IHC, and Western Blots to unravel the mechanism behind NF-kB-dependent cardioprotection following.
• Validated gene expression results from microarray and qPCR analysis, establishing novel genes involved in NF-kB-dependent outcomes.
• Employed knockout mouse models based on scientific literature to examine the mechanism of specific genes (e.g., hspa1a and hspa1b). Research Outcome
• Identified that NF-kB regulates 16 genes in NF-kB-dependent cardioprotection after Permanent Occlusion (PO) and 59 genes in NF-kB-dependent cell death after Ischemia/Reperfusion (I/R).

• Cell Culture Maintenance
• Successfully maintained and prepared various cancer cell cultures, contributing to the ongoing research in oncology.Proliferation and Apoptotic Cell Death Analysis
• Conducted analysis on human tumor paraffin sections, specifically examining proliferation using PCNA Staining and apoptotic cell death through TUNEL assays.

Ms, Molecular, Cellular, And Biochemical Pharmacology

Bs, Biology (Concentration In Molecular Biology)