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🎓 𝐌𝐚𝐬𝐭𝐞𝐫 𝐨𝐟 𝐒𝐜𝐢𝐞𝐧𝐜𝐞 (𝐌𝐒) 𝐢𝐧 𝐌𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫, 𝐂𝐞𝐥𝐥𝐮𝐥𝐚𝐫, 𝐚𝐧𝐝 𝐁𝐢𝐨𝐜𝐡𝐞𝐦𝐢𝐜𝐚𝐥 𝐏𝐡𝐚𝐫𝐦𝐚𝐜𝐨𝐥𝐨𝐠𝐲🧬 𝐀𝐒𝐂𝐏 𝐁𝐨𝐚𝐫𝐝 𝐂𝐞𝐫𝐭𝐢𝐟𝐢𝐞𝐝 𝐓𝐞𝐜𝐡𝐧𝐨𝐥𝐨𝐠𝐢𝐬𝐭 𝐢𝐧 𝐌𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫 𝐁𝐢𝐨𝐥𝐨𝐠𝐲, 𝐌𝐁(𝐀𝐒𝐂𝐏)𝐂𝐌🔍 𝐂𝐨𝐦𝐦𝐢𝐭𝐦𝐞𝐧𝐭 𝐭𝐨 𝐄𝐱𝐜𝐞𝐥𝐥𝐞𝐧𝐜𝐞: Dedicated to laboratory analyses, safety, and compliance, I’m passionate about delivering accurate results that improve patient health outcomes through innovative problem-solving and collaboration.🏢 𝐂𝐨𝐫𝐩𝐨𝐫𝐚𝐭𝐞 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐄𝐱𝐩𝐞𝐫𝐢𝐞𝐧𝐜𝐞: As a Biotechnology Associate-Scientific Clinical contractor at P&G through Integrated Resources, I isolated specific skin structures from over 200 human skin biopsies using Laser Capture Microdissection (LCM).🔬 𝐂𝐥𝐢𝐧𝐢𝐜𝐚𝐥 𝐋𝐚𝐛𝐨𝐫𝐚𝐭𝐨𝐫𝐲 𝐄𝐱𝐩𝐞𝐫𝐢𝐞𝐧𝐜𝐞: Three years in CLIA-certified molecular diagnostic labs. At Amazon Dx, we delivered precise test results within service-level agreements in a high-throughput environment processing over two million samples. At Ischemia Care, I used gene expression assays to identify cerebrovascular events, improving diagnoses and clinical treatments.📊 𝐌𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫 𝐁𝐢𝐨𝐥𝐨𝐠𝐲 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐄𝐱𝐩𝐞𝐫𝐢𝐞𝐧𝐜𝐞: Extensive experience in hospitals and academic institutions, leading to three first-author and four contributor publications, plus four posters presented at national conferences and team meetings.🔧 𝐋𝐚𝐛 𝐌𝐚𝐧𝐚𝐠𝐞𝐦𝐞𝐧𝐭 𝐚𝐧𝐝 𝐋𝐞𝐚𝐝𝐞𝐫𝐬𝐡𝐢𝐩 𝐄𝐱𝐩𝐞𝐫𝐢𝐞𝐧𝐜𝐞: Oversaw equipment procurement, trained staff and students, co-mentored a medical student, and ensured policy compliance at the University of Cincinnati College of Medicine, Cincinnati Children’s Medical Center, and Nationwide Children’s Hospital.𝑳𝒂𝒃𝒐𝒓𝒂𝒕𝒐𝒓𝒚 𝑺𝒌𝒊𝒍𝒍𝒔🔬 𝐓𝐞𝐜𝐡𝐧𝐢𝐪𝐮𝐞𝐬: Proficient in sterile techniques, Western blotting, ELISA, multiplex immunoassays, PCR, qRT-PCR, mini and maxi-preps, microarray analysis, gel electrophoresis, RNA, DNA, and protein isolation, immunohistochemistry, immunofluorescence, in situ hybridization, and laser capture microdissection.🧪 𝐒𝐲𝐬𝐭𝐞𝐦𝐬 𝐚𝐧𝐝 𝐈𝐧𝐬𝐭𝐫𝐮𝐦𝐞𝐧𝐭𝐬: Hands-on experience with the QIAcube, Affymetrix GeneChip System, whole transcriptome assays, PAXgene Blood RNA System, Hamilton Microlab STARlet Liquid Handling System, MGITech MGISP-960 Extraction Instrument, and Zeiss PALM MicroBeam LCM System.🧬 𝐒𝐚𝐦𝐩𝐥𝐞 𝐏𝐫𝐨𝐜𝐞𝐬𝐬𝐢𝐧𝐠: Expertise in processing nasal swabs, tissue samples, urine, and blood specimens.
Editage Insights (Online Academic Research And Publishing Resources)
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Invited Contributor And Author (Volunteer)Editage Insights (Online Academic Research And Publishing Resources)Cincinnati, Oh, Us
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Biotechnology Associate- Scientific ClinicalIntegrated Resources, Inc ( Iri ) Mar 2024 - Aug 2024Edison, Nj, UsContractor at P&G Global Biotechnology Department- Beauty Skin Care• Visualized skin compartments by staining prepped histological slides for Laser Capture Microdissection (LCM).• Accurately isolated stratum basale layer, super basale layers, sebaceous glands, hair roots and dermis from over 200 clinical human skin biopsies using Zeiss PALM MicroBeam LCM system. A colleague and I completed the project two months ahead of schedule• Prepared the collected fractions for RNA isolation, quality control, and RNA sequencing analysis. -
Sr Research Assistant (Lab Manager)University Of Cincinnati College Of Medicine Jul 2022 - Dec 2023Cincinnati, Oh, UsLab Management• Managed a high-profile cardiometabolic biology research laboratory.• Interviewed, trained and supervised a team of student employees. • Monitored and optimized monthly supply budget.• Coordinated the procurement process, placing orders, and maintaining cost-effective supply inventory.• Implemented and oversaw stringent lab safety protocols, ensuring compliance with university policies and federal regulations.Collaboration and Support• Provided support and assistance to fellow lab members, promoting a positive and collaborative work culture. Enhanced the overall efficiency of the research team.Mouse Colony Management• Organized and maintained a large, intricate mouse colony. • Conducted genotyping procedures for multiple mouse lines, contributing to the advancement of laboratory research goals. -
Clinical Laboratory ScientistAmazon Jan 2021 - Aug 2022Seattle, Wa, UsHigh Complexity Diagnostic Molecular Test in a CLIA, CAP, and ISO 15189 Accredited LaboratoryAccurate Test Results Delivery• Consistently delivered precise test results within stipulated service-level agreements, ensuring a customer-driven approach in a high-throughput diagnostic molecular laboratory.High-Throughput Lab Operations• Played a crucial role in processing and testing over two million samples, including comprehensive testing for SARS-CoV-2.Team Coordination and Training• Functioned as a team coordinator and member, overseeing the processing of patient samples according to Good Laboratory Practice (GLP) and Standard Operating Procedures (SOPs).• As a Trainer Ambassador, facilitated training sessions for personnel on automated liquid handling, high-throughput automated equipment, SOPs methods and procedures, and troubleshooting protocols.Equipment Maintenance and Compliance• Ensured compliance of equipment and instruments within the SOPs and accreditation standards, promptly recognizing and addressing malfunctions, and troubleshooting common errors.• Documented and escalated protocol deviations, providing valuable suggestions for improvements in the laboratory information management system (LIMS).Quality Assurance and Professional Development• Practiced Good Document Practice (GDP) and meticulous diligence, guaranteeing accurate test results and compliance with regulatory agency requirements.• Maintained an understanding of current SOPs and completed CAP Continuing Education courses, including ISO 15189 2021, Quality Culture, Root Cause Analysis, Internal Auditing, Management Review, Mistake Proofing, QMS Implementation Roadmap, Document Control, and Quality Manual Development. -
Molecular Biology TechnologistIschemia Care, Llc Jun 2019 - Nov 2020High Complexity Molecular Diagnostic Blood Test in a CLIA Certified LaboratoryRNA Isolation Expertise• Proficiently isolated total RNA from stroke patient blood using the PAXgene blood system and QIA cube. Quality control to ensure the integrity and purity of genetic material.Affymetrix Microarray Platform Execution• Executed Affymetrix microarray platform to identify the type of cerebrovascular events in clinical samples, leading to more precise diagnosis and clinical treatment. SOP Creation and Enhancement• Played a pivotal role in the creation and improvement of Standard Operating Procedures (SOPs). • Assisted in basic administrative tasks related to clinical trial data and sample management. Diagnostic Lab Management• Shared responsibilities for the maintenance, operation, and quality control duties in the clinical diagnostic lab, ensuring the highest standards of performance.• Trained new clinical technicians on equipment usage, standard procedures, and lab maintenance protocols.
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Research Assistant IiiCincinnati Children'S Hospital Medical Center Feb 2018 - May 2019UsPlacenta-Specific Gene Therapy Enhancement• Enhanced the selectivity of placenta-specific expression of growth factor DNA for gene therapy. Molecular Cloning and Specificity Determination• Determined microRNAs with the potential to express the growth factor DNA, specifically in the placenta.• Conducted molecular cloning of miRNA target sites to the growth factor DNA sequence. Transfection Studies• Transfected placenta choriocarcinoma cells (BeWo cells) with the newly isolated and purified growth factor DNA containing microRNA target sites.• Measured gene expression to validate the effectiveness of the placenta-specific gene therapy approach.Lab Operations and Safety Compliance• Managed lab operations, including ordering necessary supplies and ensuring compliance with safety protocols.• Administered a safe and efficient working environment in the laboratory. -
Molecular BiologistAdvanced Testing Laboratory Apr 2017 - Jan 2018Cincinnati, Oh, UsPharmaceutical and FDA Regulated Environment• Operated in a Pharmaceutical and FDA regulated environment at Lilly Research Laboratories. Proficient Project Execution• Delivered proficient and prompt services to successfully complete internal client projects, meeting stringent quality requirements.Mammalian Cell Culture Expertise• Maintained and troubleshot a variety of mammalian, cancer, and natural killer cell culture lines. Cell Processing for Experimental Studies• Prepared and processed cells for experimental studies and high-throughput screening, contributing to the successful execution of research initiatives.Stable Cell Line Generation• Generated stable cell lines using the lentiviral expression systems. Electronic Documentation and Notebooking• Recorded laboratory documentations and procedures through electronic notebooking, ensuring accuracy and accessibility of essential data. -
Research Associate (Neurosurgery Research)Nationwide Children'S Hospital May 2016 - Mar 2017Columbus, Oh, UsResearch Focus: Immunotoxicity in Induced Traumatic Brain Injury (TBI) Rat Model• Established the principal investigator’s laboratory, overseeing equipment and supplies determination and ordering. • Managed workplace safety, good laboratory practices (GLP), and compliance with hospital policies and federal regulations.• Contributed significantly to the development of a rat traumatic brain injury (TBI) model.• Conducted measurements of cytokines and S100b levels in lipopolysaccharides (LPS)-stimulated blood cultures of sham and rat TBI models.• Implemented behavioral tests on rats, including rotarod, Barnes maze, and open field tests.• Optimized neurological stains, including Cresyl Violet/Nissl Stain, Luxol Fast Blue, Hematoxylin & Eosin.• Demonstrated proficiency in a range of molecular biology techniques, including ELISA and tissue processing. -
Research Assistant/Associate (Nephrology Research)Nationwide Children'S Hospital Dec 2011 - May 2016Columbus, Oh, UsLab Management• Managed start-up laboratory, handling equipment and supplies determination and ordering. • Interviewed, trained, and supervised new laboratory personnel, along with co-mentoring a medical student. • Ensured highest standard in laboratory safety and compliance with hospital research policies and federal regulations.• Organized and managed laboratory mouse colony, human and mouse urine sample collections. Research Goal: Identification of Early Biomarkers for Congenital Obstructive Nephropathy (CON)• Processed both human and mouse urine for RNA isolation to accomplish quantitative PCR (qPCR), microRNA, and gene expression assays.• Performed small animal ultrasound of kidneys and bladders, isolated and micro-dissected embryonic kidneys, and bladders. Collected tissues and performed high-quality RNA isolation for qPCR, microarrays, and RNA sequence analysis. • Analyzed and executed PCR, qPCR, immunohistochemistry (IHC) immunofluorescence (IF), and in situ hybridization (ISH) for microRNA quantification and localization on tissue selections. Key Achievements• Successfully performed survival surgeries, including unilateral ureteric obstruction (UUO) and cutaneous vesicostomy.• Collaborated with other labs, leading to published papers.• Discovered that miR-205 is increased with the severity of congenital obstructive nephropathy (CON) in a mouse model.• Presented research in regional and national research conferences. • Attended 2015 Vanderbilt University Mouse Kidney Injury Workshop with direct training in UUO, cecal ligation and perforation, ischemia/reperfusion (dorsal IR and ventral IR) and 5/6 nephrectomy.
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Research AssistantUniversity Of Cincinnati Jul 2003 - Jul 2010Cincinnati, Ohio, UsResearch Focus: Nuclear Factor Kappa-B (NF-kB) in Myocardial Ischemic Insults• Designed in vivo and in vitro experiments to investigate NF-kB's role in myocardial ischemic insults, confirming its activation and determining the timing of NF-kB-dependent cardioprotection. • Employed several molecular biology techniques, for example DNA, RNA, and protein isolation from tissues, ELISA, EMSA, IHC, and Western Blots to unravel the mechanism behind NF-kB-dependent cardioprotection following ischemic preconditioning (IPC) and permanent coronary artery occlusion (PO), as well as NF-kB-dependent cell death after ischemia/reperfusion (I/R).• Validated gene expression results from microarray and qPCR analysis, establishing novel genes involved in NF-kB-dependent outcomes.• Employed knockout mouse models based on scientific literature to examine the mechanism of specific genes (e.g., hspa1a and hspa1b). Research Outcome• Identified that NF-kB regulates 16 genes in NF-kB-dependent cardioprotection after Permanent Occlusion (PO) and 59 genes in NF-kB-dependent cell death after Ischemia/Reperfusion (I/R).• Discovered that Heat Shock Protein 70.1 (hspa1b) underlies NF-kB-dependent cardioprotection after PO and NF-kB-dependent cell injurious effects after I/R.• Identified therapeutic targets suitable for clinical development in treating ischemic heart disease.• Supported lab surgeons with in vivo myocardial ischemic models.Research Dissemination• Presented research results in published manuscripts, posters at national research conferences, local seminars, and laboratory meetings. -
Research Assistant (Co-Op Student)Wood Hudson Cancer Research Laboratory, Newport, Ky May 2002 - Dec 2002Cell Culture Maintenance• Successfully maintained and prepared various cancer cell cultures, contributing to the ongoing research in oncology.Proliferation and Apoptotic Cell Death Analysis• Conducted analysis on human tumor paraffin sections, specifically examining proliferation using PCNA Staining and apoptotic cell death through TUNEL assays.
Michael Wilhide, Ms, Mb(Ascp)Cm Skills
Michael Wilhide, Ms, Mb(Ascp)Cm Education Details
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University Of Cincinnati College Of MedicineAnd Biochemical Pharmacology -
Mount St. Joseph UniversityBiology (Concentration In Molecular Biology)
Frequently Asked Questions about Michael Wilhide, Ms, Mb(Ascp)Cm
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Michael Wilhide, Ms, Mb(Ascp)Cm works for Editage Insights (Online Academic Research And Publishing Resources)
What is Michael Wilhide, Ms, Mb(Ascp)Cm's role at the current company?
Michael Wilhide, Ms, Mb(Ascp)Cm's current role is Invited Contributor and Author (Volunteer).
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What schools did Michael Wilhide, Ms, Mb(Ascp)Cm attend?
Michael Wilhide, Ms, Mb(Ascp)Cm attended University Of Cincinnati College Of Medicine, Mount St. Joseph University.
What skills is Michael Wilhide, Ms, Mb(Ascp)Cm known for?
Michael Wilhide, Ms, Mb(Ascp)Cm has skills like Molecular Biology, Cell Culture, Cell, Genetics, Science, Drug Discovery, Hplc, Elisa, Pcr, Qpcr, Molecular Genetics, Pharmacology.
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