Michelle Dewitt Email & Phone Number

Seattle, Wa, Us

Seattle, Wa, Us

New York, New York, Us

Seattle, Washington, Us

• Managed the Protein Expression & Purification team• Developed and implemented a protein expression, purification, validation, and reporting pipeline• Screened and scaled expression/purification of de novo protein biosensors via E. coli expression vectors• Co-tailored expression and purification pipeline for plate-based Golden Gate protein biosensor assemblies• Led IMAC, AKTA SEC, SEC-MALS, plate-based illumination assays, and BLI• Designed and ran functional luminescence assays to screen and identify biosensor candidates• Analyzed, interpreted, and presented data to Company; data shared with Board• Acted as point-person for issues with laboratory equipment; performed independent troubleshooting• Oversaw laboratory management and operations from February-October, 2022• Co-Developed Benchling ELN buildout; independently established standard reporting templates• Authored laboratory SOPs• Interviewed and hired Research Associates for multiple teams• Planned, purchased, and oversaw laboratory build-out

Seattle, Washington, Us

• Managed the General Protein Production Core team• Oversee project expectations, timelines, and data/results• Troubleshoot issues with contamination, solubility, expression yield, and stability • Interview FTE employees for the IPD Core Labs• Train IPD employees, graduate students (as-needed), and undergraduate research assistants on all aspects of high throughput recombinant protein expression, purification, and quantification• Optimize expression and purification processes for synthetic proteins through small- and mid-scale expression and purification screens; give tailored advice to graduate students on how to optimize protein expression for their constructs.• Consistently produce low endotoxin proteins from bacterial expression for downstream in vivo studies; independently created and optimized low endo CIP for AKTA autosampler, enabling SEC for up to 48 low endo proteins at a time.• Compile and present protein production data to internal and external collaborators

Seattle, Washington, Us

• Independently transformed, expressed, purified, and characterized 24-32 Rosetta designed proteins per week via recombinant E. coli expression vectors• Performed high throughput expression/purification screens of de novo proteins• Purified and characterized proteins via gravity and AKTA IMAC, SEC, SEC-MALS, endotoxin, and UV-Vis; exposed to CD and Octet • Co-supervised a group of 8 undergraduate research assistants• Assisted in interviewing and hiring FTE lab members and undergraduate assistants• Projects: SARS-CoV-2 mini binders, CD98 binders, chlorophyll binding dimers, FGFR2 binders, IL minibinders, and a peptide binder optimization screen

Portland, Or, Us

The Reichow Lab is an interdisciplinary structural biology lab within PSU's Chemistry Department. With the goal of understanding human disease at the molecular level, the Reichow Lab utilizes CryoEM to visualize how proteins function and interact. • Primer design, PCR, DNA transformation, Gibson cloning, site-directed mutagenesis• DNA plasmid mini-prep, PCR clean-up kits• SDS-PAGE gel electrophoresis, agarose/DNA gel electrophoresis, Western Blot• UV-VIS spectrophotometry, ultra-centrifuge, table-top centrifuge• Protein expression in E. coli• Mammalian eye lens dissection• General maintenance of lab supplies and materials (e.g., preparation of stock buffers, autoclave wet and dry materials, etc.)

Peninsula Equine Medical Center is an internationally recognized equine veterinary hospital and continuing education center. Utilizing state of the art diagnostic tools, Peninsula Equine addresses the root cause of lameness and disease. • Equine tissue dissection, biofluid extraction, animal injection• Assembly of wet/fixed mounts for microscopy, tissue/biofluid staining for microscopy• Assisted with nuclear scintigraphy, radiology, and ultrasound image acquisition• Autoclaved laboratory and surgical materials• Organized regional meetings, coordinated veterinarian schedules, oversaw duties of volunteers

Postbac, Biochemistry And Molecular Biology

Bachelor Of Science (Bs), Animal Sciences