Michelle Rozycki Email & Phone Number

Boston, Ma, Us

Natick, Ma, Us

[Contracted Leidos Employee]

Boston, Ma, Us

-Executed scale-up process development/validation experiments for 18 unique projects using the organisms Pichia pastoris, E. coli and S. cerevisiae.-Supported technology transfer of Yarrowia lipolytica to a CMO and provided on-site technical support for a 5000L manufacturing run.-Executed a material generation campaign in 1L DASGIPs using 15 unique E.coli strains to produce an enzyme used in antibiotic production.-Maintained fermentation batch records and monitored historical runs to ensure all documentation was completed by team members.-Authored and maintained operational SOPs for fermentation equipment and analytical assays.-Standardized templates for tech transfers and SOP documents as part of the Outgoing Tech Transfer Committee.-Maintained all operational and project SOPs on the quality management system Qualio as the Process Team Quality Champion.-Established a chemical inventory system for all non-hazardous and hazardous materials in a 5,000 sq. ft. shared lab space.-Represented my lab space as primary POC for all procurement and consumable requests.-Coordinated maintenance best practices for stainless & glass fermentor systems and performed routine maintenance on equipment.-Implemented 5S organization principles to streamline lab workflows for USP unit operations (Glass Reactors, Stainless Reactors, Nanodrop, YSI).

-Designed and operated aerobic E. coli fermentations in 1L DASGIP benchtop bioreactor systems.-Investigated animal-free media compositions for anaerobic Lactobacillus fermentations via shake flasks.-Established downstream purification SOPs for tangential flow filtration (TFF).-Used NanoSight-NTA particle tracking, SDS-PAGE, western blotting, and total protein assays (BCA & Bradford) to assess the quality & purity of our target protein.

Amherst, Massachusetts, Us

-Supported the course instructor in development of curriculum and course materials-Effectively communicated scientific concepts with students from diverse educational backgrounds -Prepared and delivered lectures on topics related to food science and microbiology-Supervised laboratory sessions and coordinated the use of BSL-2 organisms-Trained students in general microbiology skills, including aseptic technique

Amherst, Massachusetts, Us

-Performed in vitro fermentations of microbiome samples in a DASGIP benchtop bioreactor -Developed experimental protocols using downstream processing methods to quantify Bifidobacterium metabolite production via reverse phase HPLC -Enhanced efficiency of an enzymatic assay by reducing reagents by 50% to create a quantifiable, high throughput method-Modulated bacterial cell morphology and analyzed using scanning electron microscopy-Coordinated maintenance best practices of an Agilent HPLC system

Amherst, Massachusetts, Us

Needham, Ma, Us

-Directed the conception and development of the new food analysis laboratory established to validate product claims related to blending and vitamin degradation-Procured key lab equipment including HPLC, chemical fume hood, centrifuge-Developed key SOPs and safety protocols relevant to equipment and processes involved in vitamin measurements-Researched and developed a protocol to identify an internal quality sensory panel, which in turn established the first SharkNinja sensory panel

Dorchester, Ma - Massachusetts, Us

Although my official title was food safety intern, I held a variety of roles as the only food scientist amongst a team of chefs. My first project was standardizing and scaling a client’s recipe, a snickerdoodle-chocolate chip cookie hybrid, for retail production. I investigated a fermentation and storage issue in a fresh salsa product. I streamlined the standardization of all client recipes into baker’s percentages and units of ingredients per batch of product. At the end of the work day I assisted in processing and packaging of food products produced in the main kitchen facility.

Master Of Science - Ms, Food Science

Bachelor’S Degree, Food Science And Technology