Mike Saxton Email & Phone Number

US

South San Francisco, California, US

• Developed and performed in-vitro assays for analysis of modified T cell functionality against hematologic tumor models, including proliferation, cytotoxicity and cytokine secretion.
• Characterize cell surface phenotype of primary patient samples (bone marrow, PBMC).
• Developed ddPCR assay to track pharmacokinetics of in-vivo samples including primer/probe design, assay development, optimization, qualification and troubleshooting.
• Investigated the effects of therapeutic drugs on target induction by flow cytometry and effects on T cell function in co-culture experiments.
• Used molecular cloning techniques to produce dozens of plasmids to enable candidate selection.
• Allocated roughly 25% of time to perform lab management duties including equipment maintenance, ordering, EH&S and general organization.

South San Francisco, California, US

• Responsible for the purchase, maintenance, and repair of all lab equipment, ordering and stocking lab consumable as well as building out new lab spaces.
• Maintained gas stocks and chemical database, performed safety inspections, acted as liaison to county fire and safety departments and managed biohazard/chemical waste.
• Performed maintenance of cell lines and ELISAs for cytokine detection.

• Optimized CRISPR RNP protocol for use with hES cells and developed workflow for creating and characterizing clonal lines for diabetes disease modeling.
• Collaborated with outside researchers to bring new genome editing technologies into the lab.
• Independently plan and execute genome engineering projects (CRISPR/TALENs) as well as assist postdocs and graduate students to design genome engineering strategies tailored to their specific projects.
• Use online resources such as NCBI, Ensemble and Prosite to determine possible spliciforms, translated regions and protein domains and structure in order to discern the most effective target sites.
• Design guide RNAs, using MIT and Benchling software, as well as donor vectors and HDR template ultramers.
• Perform, troubleshoot and provide technical advice for vector design, molecular cloning, electroporation, analysis of editing efficiency (TIDE/TIDER), subcloning and clonal characterization.

San Francisco, California, US

9 month full time internship in the Conklin Lab at GICD. The lab uses human iPS cells to model cardiac genetic disease. My projects focused on engineering cell lines that express fluorescent fusion proteins which would allow the visualization of disease phenotypes in live cardiomyocytes. The process included the creation of constructs, genome editing of.

Emeryville, CA, US

Completed a 6 month part time contract working in the Lab services department of Amyris. Main duties focused on the creation of liquid and plate media with heavy focus on sterile technique and quality control. Other duties included maintaining and troubleshooting equipment and the editing and creation of SOPs.

San Francisco, CA, US

Paid research position working 24 – 40 hrs per week in the Crow lab. The lab used molecular biology techniques to answer questions related to the developmental biology and evolution of fish. My main focus was on the expression of HOX genes in the American paddlefish. Other responsibilities included the maintenance of a large zebra fish facility and the.

Bachelor Of Science In Biology

Stem Cell Technology Certificate, Biotechnology Program