Michael Germann Email & Phone Number

Durham, North Carolina, Us

Expression, purification and analysis of directed evolution CRISPR-based nucleobase editors and nucleases.

Austin, Tx, Us

Revolutionizing at-home and clinical diagnostics by initiating and leading manufacture of custom enzymes at the heart of a handheld qRT-PCR device.* Develop novel purifications of DNA polymerases and reverse transcriptases and engineer to scale.* Genetically engineer new forms of enzymes for enhanced processivity and temperature stability.* Create characterization and QC testing systems for custom enzyme formulations and blends.* Build manufacturing capabilities from scratch.

Waltham, Ma, Us

Provided enzymes for forensics, clinical diagnostics and NGS by performing and improving purification processes and QC testing in a cGMP ISO13485-regulated facility.* Purified a variety of enzymes at scales ranging from milligrams to hundreds of grams from bacteria, yeast, and mammalian sources using a wide variety of chromatography techniques (affinity, IEX, HIC), Tangential Flow Filtration and fractionation.* Developed new chromatographic processes to reduce contamination and improve yield. * Optimized purification processes to scale, saving raw material costs and reducing waste.* Automated chromatographic processes by authoring and validating new methods.* Tested manufactured enzymes by fluorometric, spectrophotometric, and radioisotope incorporation assays, HPLC, bioanalyzer, and qPCR.* Increased efficiency of enzyme assays using statistical analysis and method optimization through Six Sigma methodologies.* Implemented SAP enterprise software to manage labor and inventory as well as measure and predict customer demand and maintain Quality data.* Implemented LIMS and developed additional scheduling tools for efficient planning and communication of purification schedules to meet customer expectations.* Maintained critical instruments including equipment budget, service contracts, and routine calibration.* Onboarded, trained, mentored, and oversaw junior scientists.

The Woodlands, Tx, Us

Supported pharmaceutical discovery by purifying recombinant proteins used for in vitro screening assays and by performing assays against compound libraries.* Used molecular biology techniques (PCR, nucleic acid extraction, sequencing) to clone recombinant expression vectors for bacteria and mammalian cells.* Grew bacterial fermentations and adherent and suspension mammalian cell cultures to provide raw material for purification.* Purified recombinant proteins to be used in medium- and high-throughput screening.* Performed high-throughput screening assays in 96- and 384-well formats using automated liquid handlers and plate readers.* Metabolic analysis of animal tissue samples by HPLC.

The Woodlands, Tx, Us

* Cloned and cultured transgenic mouse stem cell lines for pharmaceutical discovery.

Developed manufacturing processes for recombinant peptides for human therapeutics.* Built plasmid libraries for optimization of protein structure and yield.* Modified bacterial expression hosts by viral gene transduction to improve recombinant protein yield and fermentation characteristics.* Prepared E. coli Master and Working Cell Banks under GMP conditions.* Performed QC testing (viral and microbial contamination, plasmid structure) on large scale fermentations under GMP conditions.