Principal Scientific Research Associate
Expression, purification and analysis of directed evolution CRISPR-based nucleobase editors and nucleases.
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Michael Germann is listed as Biochemist with extensive experience in enzyme manufacturing, molecular biology and biotechnology based in Austin, Texas, United States. AeroLeads shows a work email signal at thermofisher.com and a matched LinkedIn profile for Michael Germann.
Michael Germann previously worked as Principal Scientific Research Associate at Life Edit Therapeutics and Scientist III, Enzymology at Nuclein. Michael Germann holds Bachelor'S Degree, Biochemistry from University Of Nebraska-Lincoln.
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I am a biochemist with extensive experience in cGMP enzyme manufacturing, molecular biology and biotechnology. I have extensive skills in chromatography and enzyme assay development. I was responsible for manufacturing trusted and highly valuable enzymes at ThermoFisher Scientific for forensics, clinical diagnostics and cancer research. I was able to build upon that experience for Nuclein, where I was asked to initiate and lead enzyme manufacturing efforts for a company on the leading edge of handheld PCR diagnostics for COVID-19 and Zika viruses. My skill set includes the entire recombinant protein production process:* I can use my skills in molecular biology to clone a gene for expression into a vector.* I can transform bacterial and mammalian expression hosts and then culture cells for production of a recombinant target.* I can purify expressed targets through chromatography and TFF at a wide range of scales, from microgram to grams.* I can characterize purified targets through a multitude of techniques, such as fluorescence, HPLC, bioanalyzer and qPCR.* I can effectively document the entire process for regulatory authorities and transfer processes through development to manufacturing.
Listed skills include Cell Based Assays, Biochemistry, Molecular Genetics, Purification, and 13 others.
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Durham, North Carolina, US
Expression, purification and analysis of directed evolution CRISPR-based nucleobase editors and nucleases.
Austin, TX, US
Revolutionizing at-home and clinical diagnostics by initiating and leading manufacture of custom enzymes at the heart of a handheld qRT-PCR device.* Develop novel purifications of DNA polymerases and reverse transcriptases and engineer to scale.* Genetically engineer new forms of enzymes for enhanced processivity and temperature stability.* Create.
Waltham, MA, US
Provided enzymes for forensics, clinical diagnostics and NGS by performing and improving purification processes and QC testing in a cGMP ISO13485-regulated facility.* Purified a variety of enzymes at scales ranging from milligrams to hundreds of grams from bacteria, yeast, and mammalian sources using a wide variety of chromatography techniques (affinity.
The Woodlands, TX, US
Supported pharmaceutical discovery by purifying recombinant proteins used for in vitro screening assays and by performing assays against compound libraries.* Used molecular biology techniques (PCR, nucleic acid extraction, sequencing) to clone recombinant expression vectors for bacteria and mammalian cells.* Grew bacterial fermentations and adherent and.
The Woodlands, TX, US
* Cloned and cultured transgenic mouse stem cell lines for pharmaceutical discovery.
Developed manufacturing processes for recombinant peptides for human therapeutics.* Built plasmid libraries for optimization of protein structure and yield.* Modified bacterial expression hosts by viral gene transduction to improve recombinant protein yield and fermentation characteristics.* Prepared E. coli Master and Working Cell Banks under GMP.
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Michael Germann is listed as Biochemist with extensive experience in enzyme manufacturing, molecular biology and biotechnology.
AeroLeads has found 2 work email signals at @thermofisher.com for Michael Germann.
Michael Germann is based in Austin, Texas, United States.
Michael Germann has worked for Life Edit Therapeutics, Nuclein, Thermo Fisher Scientific, Lexicon Pharmaceuticals, and Restoragen Inc..
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Michael Germann holds Bachelor'S Degree, Biochemistry from University Of Nebraska-Lincoln.
Michael Germann is listed with skills including Cell Based Assays, Biochemistry, Molecular Genetics, Purification, Antibodies, Assay Development, Recombinant Dna Technology, and Enzymes.
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