heterologous expression of alkaline phytase in Pichia pastors;protein purification;site directed-mutagenesis to improve the enzyme propertycrystal structures of alkaline phytase

➢ Design the cloning of extracellular alkaline phytase and expression in Pichia pastoris; comparison of secretion signal peptides and optimization of expression conditions ➢ Generation and screening for multi-copy clones and determination of copy numbers with q-PCR (including extraction and purification of genomic DNA from Pichia pastoris) to investigate the effects of copy number on expression levels of alkaline phytase in Pichia pastoris ➢ Cloning and expression of intracellular alkaline phytase in Pichia pastoris; optimization of expression conditions for maximum yield and characterization of recombinant alkaline phytase by enzymatic assay and protein purification by immobilized metal affinity chromatography (IMAC) ➢ Solubilization and refolding of recombinant alkaline phytase in inclusion bodies➢ Supervised undergraduate students (more than ten) in the laboratory with individual research projects

.general chemistry lab to help students understand the materials well and guide them to perform the experiments.recitation class which was designed to help student to extend their learning from class .biomolecular chemistry lab which was designed for senior undergraduate students and I was involved in setting up the lab, designing the experiments and responsible for all the trouble shootings