• Successfully maintained stable passaging of iPSCs to cardiomyocytes daily, including media changes, replating, and freezing, while establishing and managing cell banks for both iPSCs and cardiomyocytes (CMs) to support research on heart force generation, propagation, and remodeling• Compared three iPSC differentiation protocols to promote CM growth by immunostaining CMs at various post- differentiation timepoints and assessing alpha and beta myosin expression through image analysis with Cell Profiler• Updated the lab’s standard differentiation protocol based on observed morphology and results, improving efficiency and reliability in generating CMs from iPSCs• Analyzed 20+ batches of human iPSC-derived CMs using qPCR, PCR, and western blot techniques to evaluate gene editing success and provided valuable data for ongoing research projects

• Actively contributing to the development of an innovative energy self-supplying synthetic cell project, with a focus on glucose-driven ATP synthesis• Conducting early-stage experiments to assess the functionality of Glucose Transporter embedded on the cell membrane for the construction of a functional synthetic cell

• Improved the potency of T-cell mediated killing of hematological tumor antigen presenting cells by introducing multiple co-stimulation antibodies that utilizes the surface markers as anchoring molecules• Compared and analyzed different signal 2 pathways and their dependence on signal 1 through engineered reporter assays and primary killing assays• Engineered various cell lines to express hematological tumor surface markers and validated the expression by flow cytometry