Mitchell Cheung Email & Phone Number

MOLECULAR BIOLOGY EXPERIENCE: Gene editing (Mutagenesis, fusion genes); Cloning; Primer design; PCR; Expression constructs; Vector modifications; Bacteria transformation and culturing. Total and poly A+ RNA isolation; RT-PCR. ELISA, Protein Isolation; Western Blot; Immunoprecipitation. Mammalian cell line growth and maintenance; Transfections; Creation/infection of recombinant Lentiviruses; Flow cytometry analysis; shRNA and siRNA mediated gene silencing; Cell viability assays. Mouse dissections; Colony maintenance; Genotyping.GENOMICS: Analysis of raw genomics data: WGS (whole genome), WES (exome), targeted sequencing, and RNA-seq data. Identified germline and somatic mutations: including single nucleotide variations, in/dels, and large chromosomal rearrangements. Performed differential gene expression and pathway analysis of RNA seq data from tumors and cell lines.COMPUTATIONAL SKILLS: Developed shell script workflows / pipeline for bioinformatics analysis of large genomic datasets.LABORATORY MANAGEMENT: Supervised/guided technicians and Summer students; Ordered supplies/reagents; Personally repaired or coordinated maintenance of various lab equipment; Wrote grants, progress reports, and manuscripts in collaborative studies under defined timelines; Submitted annual IRB continuation applications; Meticulously maintained laboratory notebooks and databases; Provided support for lab computer hardware and software.

Listed skills include Western Blotting, Pcr, Cell Culture, Molecular Biology, and 26 others.

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  Thomas Jefferson University

  Human Genetics