With Dr. Frank Takken. Unraveling the downstream processes of the NLR Rx1 after activation with an initial focus on DNA damageKey Activities• Project management and collaboration with Dr. M Richard• Student supervision and teaching (internships, teaching classes and practical courses)• Data visualization using R and ggplot2.• Assessor of BSc. theses (external InHolland Applied University)• Lab and greenhouse work: Setup a wide range of molecular techniqueso DNA-Damage measurements (COMET and TUNEL Assays)o Western blotting and Native Blue PAGEo Cell integrity measurements (ion leakage)o ROS measurementso Platereader assays (Autofluorescence and Quantifiable trypan blue stain protocols)o Plant transformation and tissue cultureo Transgenic N. Benthamiana creation

With Prof. Hans Thordal-Christensen. 1. Trying to identify factors that are involved in trans-kingdom RNA transfer, using Arabidopsis and the powdery mildew fungus Golovinomyces orontii. 2. Investigating paternal imprint effects in the context of Arabidopsis defense.Key Activities:• Student supervision (BSc. and MSc. Students)• Lab techniques:o Host-induced Gene Silencing (HIGS)o Q-PCRo Confocal and fluorescence Microscopyo Westernblot o Arabidopsis transformation

With Dr. Annelies Schulte. Production of economically important plant-derived compounds. Key activities:• Project Management• Plant tissue culture• Prepare plant samples for MS• Differential display to analyze differential gene expression

With Dr. Sylvia de Pater and Prof. Paul Hooykaas.Unraveling the function and evolution of the gene DAYSLEEPER (Bundock et al. Nature 2005)Key Activities:• Project management• Student supervision and teaching (supervised many students and taught courses and practical courses)• Confocal microscopy• Molecular cloning• Western blotting• Arabidopsis transformation• Tissue culture and protoplast transformation

Internship in the lab of Prof. Eric Selker under supervision of Dr. Zachary Lewis.I performed this internship in the context of the "Medical Biology" Masters program of the University of AmsterdamKey Activities:• Fungal culturing and selection (N. crassa)• Confocal Microscopy• FACS of fungal spores• PCR and southernblot

I performed an internship in the lab of Prof. Roel van Driel, under supervision of Dr. Paul Fransz and Dr. Federico Tessadori. I performed this internship in the context of the Masters program "Medical Biology" at the University of Amsterdam