Mohammed Alameri Email & Phone Number

Durham, NC, US

TCRCure Biopharma works in developing different platforms for solid cancer treatment (liver cancer, brain, lung, skin, cervical etc). We are applying different immune modalities, such as CAR-T, TCR, and NK, to target different cancer types. In addition to that, we utilize genetic tools to design better-armored immune cells.As of my role, I work in the.

Durham, North Carolina, US

• In this position, I am responsible for developing the Cas9 system from scratch to knock out Nrl gene for therapeutic purposes. The interesting part about this project is that I got to build everything from scratch.
• Design and in vitro transcription of gRNA for gene editing
• Culture and induction of bacteria for Cas9 production and purification
• Digestion of DNA with Cas9 RNPs in vitro
• Plasmid transfection of Crispr activators
• Quantification of gene expression by qRT-PCR

Durham, North Carolina, US

• The project aims to design hydrogel for T cell activation, CAR-T manufacturing, and study T cell differentiation and immune response. This is essential for adaptive cell therapy, and in vivo CAR-T Manufacturing purposes.
• Fabricated 3D Hydrogel to promote T cell activation and CAR-T production and release ex-vivo.
• Cultured T cells in 3D scaffold to understand their differentiation and immune response.
• Applied click chemistry to synthesize T cells activator antibodies conjugate to hydrogel (Ab-Tet).
• Designed flow panel to screen for specific T cell makers in flow cytometry.

Durham, North Carolina, US

• The project aims to design DropSeq technology for Single Cell RNA-seq to understand phenotypic changes of cells when cultured in hydrogel. This helped the lab in reducing the cost of ssRNA-seq.
• Designing microfluids for Single cell isolation in a droplet.
• Troubleshooting flow rate, cell, and beads concentration.
• Optimizing the operating process single cell sequencing.

Florham Park, NJ, US

1. Experience with Immune Cell Culture HSC, NK cells and T cells2. Using CRISPER to knock out several receptors from T cells and NK Cells3. Using TcBuster to conjugate Chimeric Antigen Receptor (CD19-CAR) to T cells.4. Conduct transfection and transduction for gene delivery (CD19-CAR KI, TCR KO)5. Viral Vector Production (Lentivirus) with genes of.

Florham Park, NJ, US

1. Harvesting ECM (Scaffold) from a placenta 2. Acid-Base Remediation to wash out hormones and growth factors from the scaffold 3. Quantify collagen content in the ECM using hydroxyproline assay 4. Quantify hormones presence in the tissue (hCG, Progesterone's, FSH, Estrogen, Lactenen, Oxytoxin) using Direct and competitive ELISA)5. Study the cell-scaffold.

I was in charge of conducting several testes such as ETS, PPM and CM for certain medical devices such as Ventilator, Syringe Pump, Pressure Control and Temperature Control devices.

Master Of Science - Ms, Bioengineering And Biomedical Engineering

Bachelor Of Engineering - Be, Biomedical/Medical Engineering