• Multi-step organic synthesis of small molecules, derivatives, and peptides via solution and solid phase methods using manual, automated, and microwave-assisted techniques. Synthesis, purification, and characterization of polymer/biopolymer-based biomaterials for efficient drug delivery to the target. Developed a method for synthesizing PEG-azide linker at the C-terminal of the peptide for conjugation by click reaction. • Developed multifunctional nanoparticles containing drug molecules… Show more • Multi-step organic synthesis of small molecules, derivatives, and peptides via solution and solid phase methods using manual, automated, and microwave-assisted techniques. Synthesis, purification, and characterization of polymer/biopolymer-based biomaterials for efficient drug delivery to the target. Developed a method for synthesizing PEG-azide linker at the C-terminal of the peptide for conjugation by click reaction. • Developed multifunctional nanoparticles containing drug molecules with an acid-cleavable linker, targeting peptide epitope for release in the tumor environment. Designed a synthetic route for synthesizing PEG linkers of different lengths on the targeting peptides on resin and automated the synthesis using a solid-phase synthesizer.• Developed multifunctional lipid nanoparticles (LNPs) containing mRNA and targeting antibody or diabody lipid conjugates with imaging fluorophore for CAR-T-cell therapy for pancreatic cancer. Show less

• Developed novel biomaterials and drug delivery products for Stanford faculties and researchers' collaborative projects. Helping write scientific papers, projects, protocols, and preliminary data for grant submissions. Organic synthesis, peptide synthesis, formulations, drug delivery systems, micro and nanoparticles, microneedles, protein purification, fluorescent labeling, bioconjugation, and cell culture.• Managed teams of cross-functional scientists and mentored postdoctoral and… Show more • Developed novel biomaterials and drug delivery products for Stanford faculties and researchers' collaborative projects. Helping write scientific papers, projects, protocols, and preliminary data for grant submissions. Organic synthesis, peptide synthesis, formulations, drug delivery systems, micro and nanoparticles, microneedles, protein purification, fluorescent labeling, bioconjugation, and cell culture.• Managed teams of cross-functional scientists and mentored postdoctoral and graduate student researchers.• Managed lab activities and maintained an instrumentation facility of LC/MS/MS AB-Sciex QTRAP 4000, Shimadzu analytical and preparative and analytical HPLC, CSBio peptide synthesizer, Biotage Astra Microwave organic/ peptide synthesizer. Tissue culture facility, cryostat, Fluorescence, and stereomicroscopes, Buchi mini spray dryer, Waters supercritical fluid extractor, Jasco J810 circular dichroism (CD) spectrometer, osmometer, polarimeter, AKTA FPLC protein purifications system. Nano assembler, dynamic light scattering (DLS) - particle size analyzer, microplate reader, lyophilizers, gel documentation system, and Wave bioreactor culture system. Show less

• Developed small molecule incorporated thermoreversible gel formulations for delivering into the gastrointestinal tract. • Optimized drug encapsulation, microparticle size, excipients, gelation temperature, and physicochemical properties.

• Developed chelators incorporated transdermal patch formulations for accelerated wound healing.• Small molecules incorporated pullulan-based hydrogel patch for scarless wound healing.• Analysis of the formulations by ex vivo skin penetration using Franz cell, UV-Vis, HPLC, LCMS, and ICP-MS.

• Organic synthesis of small molecules and peptides via solution and solid phase methods using manual and automated techniques. Synthesis, purification, and characterization of polymer/biopolymer-based biomaterials for efficient drug delivery. • Biophysical characterization of drug molecules using HPLC, LC-MS, and NMR. Pharmacokinetics, pharmacodynamics by LC/MS/MS analysis. Study of interactions between proteins and small molecules. Linking pharmaceutical compounds to biopolymers for… Show more • Organic synthesis of small molecules and peptides via solution and solid phase methods using manual and automated techniques. Synthesis, purification, and characterization of polymer/biopolymer-based biomaterials for efficient drug delivery. • Biophysical characterization of drug molecules using HPLC, LC-MS, and NMR. Pharmacokinetics, pharmacodynamics by LC/MS/MS analysis. Study of interactions between proteins and small molecules. Linking pharmaceutical compounds to biopolymers for sustained/targeted delivery. Distribution of drug molecules in a film/patch and gel formulations. Secondary structure, self-assembly, and aggregation of peptides, proteins, and other biomolecules. • Developed collagen-based controlled release delivery system by conjugating growth-factor mimicking peptides to improve the survival of stem cells implanted into organs.• Developed biomaterials for drug delivery, such as transdermal patches, creams, suspensions, and injectables. Testing of various types of pharmaceutical and biopharmaceutical formulations for controlled drug delivery. Targeted delivery to organs, sustained drug delivery using micro and nanoparticles. Show less

• Self-assembly, conformation, and kinetics of aggregation/oligomerization, fibril formation, morphology, and cleavage pattern/kinetics of limited proteolysis of a Japanese variant (ΔE22) of Amyloid β-protein.• Mapping key regions and residues that control Amyloid β-protein oligomerization, aggregation, and fibril formation by double/single-D-aminoacid substitutions. Studying 20/22 Aβ peptides with double-D-enantiomer substitutions at different positions in the sequence of each of Aβ (1-40)… Show more • Self-assembly, conformation, and kinetics of aggregation/oligomerization, fibril formation, morphology, and cleavage pattern/kinetics of limited proteolysis of a Japanese variant (ΔE22) of Amyloid β-protein.• Mapping key regions and residues that control Amyloid β-protein oligomerization, aggregation, and fibril formation by double/single-D-aminoacid substitutions. Studying 20/22 Aβ peptides with double-D-enantiomer substitutions at different positions in the sequence of each of Aβ (1-40) and Aβ (1-42) for the initial scan. Further detailed studies using peptides with single-D-aminoacid substitutions.• Concentration effects of cosolute trimethylamine-N-oxide on conformation, morphology, and assembly dynamics associated with key intermediates in Amyloid β-protein fibril formation. Show less

• Studying conformational changes of amyloid-forming peptides and the effect of osmolytes on their aggregation.• Solid phase peptide synthesis: Synthesized Aβ, Polyglutamine, IAPP, and Prion peptides and their derivatives of various lengths. Fluorescent labeling of peptides for FRET studies.• Biophysical and biochemical studies on folding and self-assembly of peptides in different solution conditions using circular dichroism, FT-IR, and fluorescence spectroscopic techniques.