Perform IHC and ISH using Leica Bond automated system Help to develop and deploy a new tissue inventory system to maintain samples

• Perform mRNA production, specializing in the upstream process of in vitro transcription of DNA into mRNA using commercial and custom automation• Extensive operator knowledge of Hamilton Liquid Handlers, HighRes automated system and of formulating mRNA by using a nano assembler• Attended Hamilton Venus beginner method development training and HighRes Cellario training • Assisted in operationalizing the automated HighRes Biosolution system that runs the small scale process • Enhanced efficiency of lab operations by changing the cadence of the reactions between shifts to decrease turnaround time from 42 days to 28 days• Contributed to creation of batching rules for mRNA production to cut down on costs and decrease turnaround time by efficiently categorizing mRNA into different scales • Performs daily batching of all mRNA orders that come through production to determine the appropriate train for each order to ensure a quick turnaround time • Analyze Electropherograms of mRNA from a Fragment Analyzer to determine the purity and length of each order• Developed and maintained SOPs for the small scale process• Runs routine maintenance of automated and custom equipment

• Performed Reverse Phase Chromatography on AKTA explorers• Presented at Moderna’s first Poster Session • Attended training on unicorn software by GE• Helped build-up the small scale process resulting in a 2.5x increase in capacity • Perform oligo dT purification on a Hamilton as well as manual manifolds • Perform routine quantitation using a Biotek reader and the BMG SPECTROstar reader