Mona Riyahi Rad Email & Phone Number

Executed Sanger sequencing to analyze RNU4-2 mutations in biological samples, achieving high accuracy in mutation detection and data quality. Designed and optimized primers tailored to specific genetic targets, enhancing the precision and reliability of sequencing outcomes. Conducted gene expression and mutation analyses on C9orf72 samples, utilizing DNA fragment analysis, gel electrophoresis, and capillary electrophoresis to characterize repeat expansions. Interpreted sequencing and fragment analysis data to reveal gene regulatory and mutational patterns, advancing neurogenetics research. Refined DNA extraction and purification methods from biological samples, including blood and brain tissue, ensuring robust and reproducible results in downstream applications.

Brighton, England, United Kingdom

In this project, we focused on the application of CRISPR-Cas9 and CRISPR-Cas12 technologies to induce targeted breaks in the ACTB and GAPDH genes in mouse embryonic stem cells (mESCs). The primary objective of this study was to evaluate the differences in DNA repair pathways, specifically HR, MMEJ, NHEJ.Techniques Employed:Gene Cloning: Constructed vectors expressing specific guide RNAs, ensuring high targeting efficiency and minimal off-target effects.Cell Culture: Maintained and prepared mESCs under optimal conditions to ensure their pluripotency and viability for transfection.Transfection: Employed state-of-the-art transfection methods to introduce CRISPR-Cas systems into mESCs, achieving high transfection efficiency.Genomic DNA Isolation: Isolated high-quality genomic DNA from transfected cells, essential for precise downstream analysis.PCR and Gel Electrophoresis: Amplified and visualized target gene regions to confirm successful gene editing and to assess the editing efficiency.DNA Bead Purification: Utilized DNA bead purification to obtain clean PCR products for accurate sequencing and further analysis.Restriction Enzyme Digestion: Performed restriction enzyme digestion to verify target cleavage and to estimate the efficiency of gene editing.Guide RNA Design: Developed specific guide RNAs for targeting ACTB and GAPDH genes, optimizing them for maximum efficacy and specificity.TIDE Analysis of Sanger Sequencing: Conducted TIDE (Tracking of Indels by DEcomposition) analysis on Sanger sequencing data to assess the efficiency and nature of induced mutations, providing insights into the predominant repair pathways.

Iran

Animal Models and Experimental Techniques:Ischemia & Hemorrhage Models: Developed and applied rodent models for ischemia and intracerebral hemorrhage studies.Stereotactic Surgery: Performed precise stereotactic surgeries, including cannulation, on rodent brains.Blood Sampling & Gavage: Skilled in blood sampling (eye, tail, heart) and gavage for substance administration in rats.Cardio-Cerebral Perfusion: Conducted cardio-cerebral perfusion to study circulatory and cerebral systems.Histological and Stereological Techniques:Fixation & Staining Methods: Applied fixation techniques and staining methods, including TTC, H&E, Nissl, for tissue and cell morphology.Microtome & Sectioning: Expert in tissue sectioning using microtomes for precise histological analysis.Oxidative Stress Testing: Conducted assays like MDA and Thiol to measure oxidative stress markers.Laboratory Animal Research:Surgical Procedures: Experienced in injections, anesthesia, stroke surgeries, and tumor induction in rodents.Physiological Analysis: Conducted analyses involving gavage, handling, and restraining techniques in rats.Behavioral Studies: Developed hippocampal models and performed behavioral tests like Morris Water Maze, Open Field, and Forced Swimming. Familiar with electrophysiology.Record Keeping: Maintained and evaluated comprehensive animal records for experiments.

Gene Discovery Research:Conducted extensive research on gene discovery related to monogenic disorders and complex traits.Utilized advanced genetic techniques to identify and characterize novel genes associated with specific phenotypes and diseases. Recruited families with hereditary deafness for genetic studies.Collected detailed family histories, clinical data, and biological samples in collaboration with clinicians and genetic counselors.Genome Sequencing:Performed whole genome sequencing (WGS) on samples from recruited families to identify genetic variants associated with hereditary deafness.Analyzed sequencing data using bioinformatics tools to pinpoint mutations and variants of interest.Key Responsibilities:Managed collection, processing, and storage of biological samples, maintaining sample integrity.Analyzed sequencing data and identified pathogenic variants using bioinformatics tools.Worked with researchers, clinicians, and genetic counselors to integrate genetic findings into clinical practice.Contributed to scientific publications findings at conferences on gene discoveries and genetic disorder research.

Mashhad, Khorasan, Iran

Molecular Genetics:Conducted advanced molecular genetic techniques including PCR, RT-PCR, QF-PCR and Gel Electrophoresis for various genetic analyses.DNA Extraction:Performed high-quality DNA extraction from diverse biological samples, ensuring sample integrity for subsequent applications.Cytogenetics:Gained foundational knowledge and practical experience in cytogenetics, focusing on chromosomal aberrations.Proficient in karyotyping for diagnostic purposes, involving cell culture techniques, peripheral blood culture in media, G-Banding, slide preparation, and harvesting.

Mashhad Pathobiology Lab. Mashhad, Iran

Hematology, Biochemistry, and Hormones:Conducted comprehensive analyses in hematology, biochemistry, and hormone testing, including Sysmex operations, ESR tests, VDRL tests, and PRP tests.Immunology and Serology:Performed detailed immunology and serology tests to detect antibodies and antigens, contributing to the diagnosis of infectious diseases, autoimmune disorders, and allergies.Reception and Blood Sampling:Managed reception duties and coordinated blood sampling for both outpatients and hospitalized patients, ensuring efficient and accurate collection processes.

Mashhad County, Razavi Khorasan Province, Iran

Hematology and HormonesHematology: Conducted blood analyses to evaluate health, diagnose diseases, and monitor conditions, including complete blood counts (CBC), blood smears, and coagulation tests.Hormones: Executed assays to measure hormone levels, crucial for diagnosing endocrine disorders, reproductive issues, and metabolic diseases.Blood and Urine CultureConducted microbiological cultures of blood and urine to detect infections and determine pathogen susceptibility to antibiotics, aiding in treatment selection.Biochemistry, Immunology, and SerologyBiochemistry: Analyzed blood and fluids to measure glucose, lipids, enzymes, and electrolytes.Immunology and Serology: Conducted tests to detect antibodies and antigens, providing insights into immune response and diagnosing diseases.Technologies UtilizedCobas: Automated system for precise clinical chemistry and immunoassay testing.ELISA: Technique for detecting and quantifying proteins, antibodies, or hormones.ECLIA: Sensitive method combining immunoassay principles with electrochemiluminescence.Autoanalyzer: Automated analyzer for efficient biochemical and immunological testing.

Master Of Science - Ms, Genetic Manipulation And Molecular Cell Biology, Merit

Skills: Written Communication • Clinical Development • Pharmaceuticals • Proactive Leadership • Attention to Detail • Standards.

Bachelor Of Science - Bs, Biotechnology, 3.10 Out Of 4