Ryan M. Email & Phone Number

Gillette, Wy, Us

Brisbane, California, Us

Coordinate high-level initiatives to increase efficiencies in the implementation and support process for an enterprise level transplantation LIMS. Revise and construct standard operating procedures (SOPs) for software implementation, demonstration, and support while building a team for maintenance of SOPs across a wide range of clients. Integrate each clients’ processes and preferences into cohesive workflows during software implementation and provide consultation related to operations to minimize disruption as a result of any changes. Provide technical expertise for the creation, maintenance, and support of existing and new software development and data migration efforts.● Maintain and continually improve implementation and support protocols and processes.● Provide client-facing software training, support, and workflow optimization.● Construct and maintain clinical and operational reports across all clients.● Built production grade, database agnostic R Shiny library for the automated analysis of Single Antigen Bead (SAB),epitope identification/analysis, on-the-fly amino acid alignments, and pre-/post-transplant risk assessment.

Philadelphia, Pa, Us

Philadelphia, Pa, Us

• Perform Labtype sequence specific oligonucleotide (SSO) testing, Olerup sequence specific primer (SSP) testing, Labscreen single antigen testing, bone marrow engraftment monitoring, living and deceased donor complement dependent cytotoxicity (CDC) and flow cytometric crossmatching, and next generation sequencing (NGS) using the Illumina MiSeq platform• Develop and implement laboratory standard operating procedures (SOPs)• Designed and validated semi-automated methodology for performing virtual organ transplant crossmatches (VXM)- Greatly reduced transcription errors and enhanced data analysis capabilities- Simplified process for being performed remotely- Implemented as a clinical decision making tool for laboratory personnel and physicians for deciding if organs should be accepted for transplantation• Developed, implemented, and validated automated NGS protocol using Hamilton Robotics STARlet instrument for NGS pre-PCR, quantitation, pooling, normalization, and library preparation• Collaborate with laboratory personnel to manage quality improvement plans and projects

Seattle, Wa, Us

• Lead technical operations of clinical Biochemical Genetics Laboratory • Perform lysosomal enzyme testing, amino acid analysis, acylcarnitine profile testing, and organic acid analysis• Develop and implement laboratory Standard Operating Procedures (SOPs)• Manage operation and maintenance of high pressure liquid chromatography (HPLC), tandem liquid chromatography mass spectrometer (LC-MS/MS), spectrophotometer, and gas chromatography mass spectrometer (GCMS) analyzers• Managed validation of GCMS analyzer• Developed methods for automation of organic acid data analysis and result entry- Organic acid analysis automated by computer macro that uses logic to pre label peaks- Reduced data entry time for some assays by greater than 75 percent• Collaborated with Lab Supervisor to manage quality improvement plan and CAP accreditation

Seattle, Wa, Us

• Performed lysosomal enzyme testing, amino acid analysis, acylcarnitine profile testing, and organic acid analysis- Participated in validation and implementation of quantitative serum methylmalonic acid analysis by LC-MS/MS• Communicated with clinicians regarding test methodology and patient results• Identified and investigated instrument and assay failures• Led process improvement activities - Decreased maple syrup urine disease assay run time by 50 percent- Increased the number of detectable amino acids in amino acid assay- Increased longevity of muccopolysaccharide reagents through reagent stability studies from 24 hours to 30 days

Seattle, Wa, Us

• Performed routine laboratory testing including automated chemistry, urinalysis, and hematology procedures, including but not limited to: automated CBC differentials, blood smear morphology/counts, coagulation, and body fluid analysis• Led the validations of body fluid linearity and analytical measurement range testing• Designed and implemented improvements of urinalysis bench to improve patient safety and reduce costs• Developed a Tableau dashboard method to track hemolyzed specimens, used data to educate providers in the emergency department and reduce recollection frequency of pediatric blood draws

Seattle, Wa, Us

Worked independently and collaborated with Oakridge National Lab to quantitate and differentiate bacterial quorum sensing molecules by HPLC and biosensor assays

Seattle, Wa, Us

• Conducted an independent research project (supervised by a postdoctoral fellow) involving molecular techniques and qRT-PCR• Presented data at laboratory research meetings

Bachelor Of Science (B.S.), Microbiology, General

Bachelor Of Science (B.S.), Clinical Laboratory Science/Medical Technology/Technologist