Marcus Bezerra Email & Phone Number

Eusébio, Ceará, Brazil

• Design of plasmids for recombinant expression of monoclonal antibodies and antibody fragments
• Generation of candidate molecules (mAbs and therapeutic enzymes) for biopharmaceutical development
• Characterization of new anti-CD19 and anti-CD20 mAbs for immunotherapy via protein engineering techniques
• Development and discovery of human monoclonal antibodies using soluble proteins and whole cells
• High throughput analysis of mAb sequences by NGS (Illumina®)
• Elaboration of kinetics assays (BLI, Octet®), nano-DSF and flow cytometry (staining and functional assays, apoptosis, ADCC and CDC) of monoclonal antibodyes (IgG) and their fragments (scFv and scFv-Fc)

Fortaleza, Ceará, Brazil

• Selection of monoclonal antibodies for immunotherapy using synthetic peptides and whole cells using phage display
• Amplification of variable domains (heavy and light chain) for scFv library construction
• Development of protocols for analyses of variable domain sequences obtained via phage display by next-generation sequencing (Illumina®)
• FastQ analysis of antibody sequences by ATTILA (AuTomated Tool for Immunoglobulin Analysis) to propose enriched candidates from phage display campaigns
• Recombinant expression of scFv candidate molecules in prokaryotic platform (Escherichia coli)
• Implementation of the eukaryotic recombinant platform (ExpiCHO and Expi293)

Fortaleza, Ceará, Brazil

• Directed evolution, via phage display, of one therapeutic monoclonal antibody
• Obtention of five anti-CD20 scFv candidates
• Implementation of the phage display and prokaryotic recombinant expression platforms
• Establishment of protocols for phage display selection of antibodies
• PCR, cloning, sequencing, recombinant expression and characterization of functional variants of one therapeutic antibody developed by directed evolution
• Recombinant expression yield improvement in prokaryotic platform of scFv molecules from <1mg/L to >4mg/L

Fortaleza, Ceará, Brazil

• Project and grant writing
• Establishment of three molecular diagnostic tests (PCR) for animal health
• Co-approval of two grants (R$230,000.00) for the molecular biology lab structuring
• Formal project presentations to funding agencies

Solna, Stockholm County, Sweden

Stockholm, Stockholm County, Sweden

• Obtention of protein mutants via site-directed mutagenesis
• Functional characterization of mutants by in vitro assays (actin polymerization assay) and mass spectrometry (protein disorder analysis)
• Actin quantification of mutant cell lines by flow cytometry and confocal microscopy
• Functional characterization of anti-CD20 antibody mutants by flow cytometry
• Co-supervision of master student on their thesis “Molecular Characterisation of WASp Patient Mutations to understand Actin Regulation in T cells”, Master of Science da University of Applied Sciences (FH Campus Wien.

Fortaleza, Ceará

• Establishment of protocols for phage display library constructions for directed evolution
• PCR, cloning and recombinant expression of antibody fragments
• Establishment of protocols for epPCR library construction and diversity analysis of repertoire via Sanger and NGS (Illumina®)
• Obtention of one library of mutants aiming directed evolution and affinity maturation of one therapeutic antibody

West Point, PA

• Development of automation capable protocols for cell assays and bioprocess characterization (>99% reproducibility)
• Optimization of protocols for cell detachment from microbeads, counting/viability and flow cytometry (cell cycle)

Fortaleza Area, Brazil

• Clinical and molecular profile of MEN1 patients
• qPCR and genotyping

Doctor Of Philosophy - Phd, Biotechnology

Engineering of monoclonal antibodies towards affinity improvement. Recombinant expression of antibody fragments (scfv, scfv-Fc and IgG1)..

Doctor Of Philosophy - Phd, Biotechnology

In vitro assays (flow cytometry, actin polymerization, apoptosis). Recombinant protein expression. Site-directed mutagenesis.

Mestrado Em Biotecnologia De Recursos Naturais, Biotecnologia

Antibody engineering (directed evolution and rational design) at Fundação Oswaldo Cruz

Bachelor'S Degree, Biotechnology

Activities and Societies: Junior enterprise, Outreach programs, Summer/Winter internship. Exchange year at Boston University.Final.

Bachelor Of Biotechnology, Biotechnology

Bachelor'S Degree, Biotechnology