Meagan Tomasso Email & Phone Number

Philadelphia, Pennsylvania, United States

Recent PhD | Expert in Characterizing Protein-Protein Interactions and Dynamics | Lead Researcher at Padrick Lab• Developed skills using Geneious™, bioinformatics software, for vector and DNA primer design, as well as DNA and protein sequence analysis• Performed RNA/DNA isolation, purification, and analysis using agarose Gel Electrophoresis• Achieved skills in DNA/RNA analysis, manipulation, molecular cloning, and quantification using both PCR and RT−qPCR techniques.• Generated and implemented several analytical assays using Fluorescence Anisotropy/Polarization [ for steady state and kinetic analysis], and Analytical Ultracentrifugation Techniques. • Achieved proficiency in both bacterial and mammalian cell culture.• Adept at protein expression, conjugation, purification, and analysis. • Experience in the generation of both fusion/chimera proteins such as GFP and Maltose tagged proteins, as well as the generation of conjugated proteins via maleimide and succinimide directed reactions. • Protein Purification Techniques include Fast Protein Liquid Chromatography – AKTA Systems, Gravity Methods of Protein Purification, Affinity Chromatography, Ion-Exchange Chromatography, Size Exclusion/Gel Filtration Chromatography, Desalting and Protease Mediated Cleavage of Protein Tags.• Protein Analytical Techniques include Polyacrylamide Gel Electrophoresis, Western/Immuno-Blotting, Protein Quantification Using Spectrophotometer & Nanodrop, Hydrodynamic Radius Analysis, and Degree of Labeling Calculations of Fluorescently Label Proteins. • Developed skills in Epi-Fluorescent microscopy, Total Internal Reflection Fluorescence (TIRF), and Confocal Microscopy• Developed skills in python for data analysis as well as Microsoft Office Suite, and Adobe Suite for figure generation.• Developed experience using electronic lab notebooks, both Lab Archives and Benchling.

Doctor Of Philosophy - Phd, Biochemistry

PhD candidate Dissertation: Cdc42EP3-bound septin filaments promote actin filament assembly

Bachelor'S Degree, Biochemistry