Mrunal Borgave Email & Phone Number

Seattle Metropolitan Area, Washington, United States

1. CD3 Ab decoration of LNP for cell type targeting A. Deacetylation of SATA-modified protein to generate Sulfhydryl group B. Perform Ab conjugation using Maleimide reaction C. Purification of thiol-Ab using size exclusion column 2. GalNac decoration of LNP for liver targeting 3.Encapsulation of mRNA and ssDNA with different ionizable lipids (SM102,ALC0315,LP01) 4. Purification of LNP by dialysis and tangential flow filtration (TFF) 5. Annealing of ssDNA 6. Analysis of mRNA concentration by Ribogreen assay

• Soluble and Insoluble Expression of single domain antibody (VHH) in E. coli expression system• Cloning, Phage amplification, Finding new antibodies by Phage display method, Phage ELISA• Purification of various VHH antibody using Affinity chromatography (AKTA system)• Solubilization and refolding of VHH antibody and its drug conjugates• Preclinical animal studies• Target identification of antibody using the western blotting and MS/MS analysis• Development of diagnostic device for neonatal sepsis (Funded by Bill and Melinda Gates foundation)• Endotoxin analysis• Knowledge of large-scale production using fermentation technique • Compilation and presentation of data in written and oral formats

Pune, Maharashtra, India

• Standardized a diagnostic duplex PCR assay for screening of Mycoplasma gallisepticum and Mycoplasma synoviae.• Mycoplasma screening from poultry samples

Pune , India

Thesis Title: Microbial Cell Factories for Bioconversion of Glycerol into Value added productsObjectives:1. Identification of the key enzyme responsible for converting the bio glycerol into value added products in Klebsiella pneumoniae and Enterobacter aerogensb. Isolation of potential thermophilic strain for an efficient and economical bio glycerol conversion into value added products like 1,3 PDO and 2,3 BDO

Master'S Degree, Biotechnology, A+

Bachelor'S Degree, Biotechnology, A+