Vaccine Bulk Manufacturing :-Pertussis 5 components protein vaccine Bulk manufacturing up to 4000L (downstream) and in process testing-Diphtheria toxoid vaccine bulk manufacturing upto 1200 L (upstream)

• Analyzed vaccine and protein drug product, drug substance, and Bio-similar products using HPLC (SEC, CEX, RP-HPLC) for Purity, aggregation , impurity and identifications (under GLP and cGMP conditions)• Performed method qualification and validation• Wrote protocol, method and validation reports • Used LIMS for laboratory results managements and Empower to analyze HPLC integrations•Prepared buffer and mobile phase •Characterized process using bio-analytical tools : pH meter, Osmometer, Opalescenc, particle count

• Antigen downstream process development and scale up/down 2 L to 200 L: Bioreactor harvest filtration by different filter media• Virus purification using different chromatography• Ultrafilatrtion and diafiltration (UF/DF) by Tangential flow filtration (TFF)• Wrote Batch production record (BPR) and SOP• Analytical methods for process characterization: SDS-PAGE, Western blot, extraction of RNA, qPCR, quantify DNA

To provided health, safety, and social support for Globalink Research Interns (GRIs) from Brazil, China, France, India, Mexico, Saudi Arabia, Turkey, and Vietnam. Organized social events and act as an emergency contact for interns during their stay in Canada.

•Lab demonstrator for undergraduate chemistry students (Courses: Chemical structure, chemical dynamics, Introductory Biochemistry) •Guided students for their experiments, colorimetric titration, organic and inorganic analysis and grade lab reports•Evaluated exam papers with attention to detail and report course grades to the course director following tight deadlines•Organized and monitored exams of more than 700 students to ensure integrity of exams

Manage peer mentors and organize events for York International at York University.

•Molecular biology techniques (DNA/RNA isolation, cloning, PCR, q-PCR)•Protein expression optimization •Protein purification optimization (His tag, GST tag, Ion exchange column, size exclusion column, HIC by AKTA FPLC)•Assay development, validation of developed assay under GLP working condition•Process characterization, sample analysis, method development using bio-analytical tools including HPLC, SDS PAGE, Western blot•Protein-Protein, Protein Ligand interactions by Isothermal Titration Calorimetry (ITC), pull down assay, In-vivo and in-vitro work on S aureus bacteria•Isolation of Wall Teichoic Acid from S aureus and analysis by NMR (Bruker 700)•Analytical instrument calibration •Supervise undergraduate students research•Data analysis, interpretation and result presentation in group meetings

•Worked on the production of thrombolytic drug ‘reteplase’ (cloning and expression in P.pastoris )•Molecular biology techniques •Protein expression optimization, purification optimization •Aseptic sampling for fermentation process optimization •Amino acid scale up fermentation by 5 L and 50L bio-reactors •Fermentation media optimization

•Conduct external and internal audits; prepare audit reports according to local and global regulatory authorities.•follow up corrective and preventive actions (CAPA) and audit findings according to cGMP and ICH guideline•Write, review, train and update standard operating procedure (SOP)•Worked on the site master file (SMF) preparation, review and update according to quality management guidelines•File market complaints and conduct interdepartmental investigation (production, QC, supply chain, R&D)