µTAS and Optical Microsystems

Scientist at Microsystems Laboratory EPFL & Bio-Microfluidics Engineer at NagiBioScience SAOrganism on Chip Industrialization • Fabrication of the microfluidic chips using soft lithography (glass/PDMS), hot embossing, and other microfabrication techniques at the EPFL Center of MicroNanotechnology (CMi)• Solved and improved connectivity and the integration of the PDMS and Rigid Chips to the robotic platform and is responsible for the fabrication of existing microfluidic/fluidic integration with the platform. • Integrated the sensing elements into the chips and device for biological studies (Oxygen). Defined and implemented, monitoring and diagnostic tools (flow, pressure)• Performing biological experiments using the microfluidic rigid chips and testing their feasibility on the robotic platform as a PDMS alternative. • Developed Calibration, implemented testing protocols, and upgraded fluidic routines.• Industrialization fluidics connectivity selections.• Assisting CTO in getting CE Approval & Supply of microfluidics resources risk assessments• Trained Engineers & Biologists.

Liver On-Chip systems:• Developed liver cell line 3D culturing chip with the induction of alternative material(PDMS free). Demonstration of Liver cancer cell line culture and disease modelling. • Optimized bioassays for microfluidics chip for cell viability and functional analysis • Co-developed PDMS based large scale Three-dimensional co-culture of liver cancer cell line and on chip differentiation of iPSCS into hepatocytes. • Developed Hybrid Bioprinting aided Liver On chip device, conference paper at MicroTAS2020. Sarcoma Cancer Cell Migration: • Developed microfluidics device for Sarcoma cell time-lapsed fluorescent monitoring.

Acousotophoresis and inertial microfluidics-based sample preparation [cells sorting, separation, filtration, and isolation] from bio-fluids for point of care diagnostics: • The sample preparation platform for Sepsis developed using inertial µfluidics technique and successfully demonstrated bacteria separation from whole blood. • µBubbles Activated cell sorter (BACS) developed using acoustophoresis technique and microbubbles and successfully demonstrated its ability to sort cancer cells.• Microflow cytometer developed using elasto-inertial technique based cell focusing in an integrated µcapillaries- optical fiber to successfully present microflow cytometer in collaboration with an optical expert. • Size based MicroFiltration Assisted in the development of dean flow-based inertial cell filtration U shaped device and successfully demonstrated blood cell and cancer cell filtration.• Acoustic sample preparation platform for lower tract respiratory infections Acoustic trapping used with antibody functionalized silica particle in glass micro-capillary to develop a platform for European union project (FP7) RAPP-ID. Bacteria enrichment was successfully demonstrated. • Sample preparation for bloodstream infections assisted in µfluidic method development to lyse blood cells while extracting bacteria for downstream analysis.Teaching/Tutoring:• MS students Lab sessions, selected lectures and evaluation of course ‘Micro & Nanotechnology for Biotechnology’• Conducted inertial microfluidics theoretical and laboratory sessions forNordic Five Tech N5T summer school in Stockholm• MS Thesis Supervision and mentoring

Investigation on the use of non-linear ultrasound for the osteoporosis diagnostics.

Installation, maintenance, troubleshooting and service of equipment at centralised Lab facility. Flow cytometer, Scanning Electron μscope with EDS, Florescent-Phase Contrast μscope, GeneticDNA Analyzer, PCR Cycler, X-Ray Diffractometer, Atomic Absorption Spectrometer.

Adviser to technical manager and trainer for installation and services of molecular diagnostic lab equipment. Sales technical briefing to clients and sales team.

Installation, maintenance, and services of molecular diagnostic equipment e.g. real time PCR machines.Technical sales briefings