Mukesh Maharjan, Ph.D. work email
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Mukesh Maharjan, Ph.D. personal email
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• I am a research scientist with a very strong background in Molecular Biology, Biochemistry, Genetics, and recently in Immunology with 7+ years of interdisciplinary research expertise. Passionate for innovation as evidenced by the development of an optimized in Vivo model animal cloning tool, 3 scientific publications in peer-reviewed journals, 6 awards & scholarships.• Excellent in written and oral skills with the ability to simplify complex scientific information as demonstrated by delivering 250+ lectures in the university, leading to a teaching award and 10+ talks & presentations (including an international level conference).• Can adapt to the changing environment. I had to change my major project after the 4th year of Ph.D. and successfully completed it with 3 research papers within less than 3 years.Key technical skills:• Molecular biology techniques (ddPCR, qRT-PCR, vector design, cloning, EMSA)• Biochemical techniques (Protein expression in E. coli and insect cells/ purification, western blot, ELISA, mass spectrometry Data Analysis)• Flow cytometry/ cell sorting• Cell culture (primary and cell lines; mammalian and insect)• Statistical data analysis (GraphPad-Prism, JMP, FLow-Jo, MS-excel etc)• Team lead, project management.• With a strong background in basic science, I am motivated in bridging it with translational research to accelerate drug product formulation/development. For that reason, I am actively looking for R&D Scientist level opportunities in the industry, where I can apply my technical expertise and enthusiasm to contribute effectively to the progress of the team while making some positive changes in the society.✔ Let's connect and have a chat to understand each other. A 5 min talk is worth more than 10,000 words.email: mukkucha@gmail.com
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ScientistTakara Bio Usa, Inc.United States
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ScientistTakara Bio Usa, Inc. Oct 2021 - PresentSan Jose, California, Us -
Postdoctoral Research AssociateMd Anderson Cancer Center Sep 2020 - PresentHouston, Tx, Us• Perform and design experiments that involve lung stem cell transplantation, flow cytometry, immunohistochemistry/fluorescence, FISH, confocal microscopy, organoid assays.• Optimize COPD (Chronic Obstructive Pulmonary Disease) mouse model, to see the recovery and chimerism of disease damaged lung through stem cell transplantation.• Worked with elastase induced COPD mouse model and track and treat the COPD progression by continuously micro-CT scan data analysis and stem & progenitor pulmonary cell transplantation.• Independently design and optimize organoid swelling assay for in vitro verification of the recovery of the Cystic Fibrosis mouse model in 3D Matrigel culture.• Worked on the generation of Veto T-cells. Multiplying Veto cells and studying the tolerance developed by it in the successful allogeneic transplantation of lung stem cells. -
Postdoctoral Research AssociateBaylor College Of Medicine Sep 2019 - Sep 2020Houston, Texas, Us▸Lead a project by designing and planning experiments that involve bone marrow transplant, Flow cytometry, behavioral testing, primary cell culture, gene copy no. analysis by ddPCR to elucidate the links between stem cell function and neurodegenerative disease ▸Cross-functional collaborations with different labs and conduct live or virtual meetings; direct the project by adapting to the changes that are needed for its successful completion.▸Extensive reading and writing of the latest scientific literature that resulted in a manuscript for publication and grant proposal of future research. ▸ I use tools which include the following techniques on top of general Molecular biology and Biochemistry and genetics: ▸ Flow Cytometry ▸ Cell Sorting ▸ Cell culture/ Primary cell culture ▸ Bone marrow transplant and chimeric mouse ▸ ddPCR data analysis ▸ qPCR, CBC, genotyping, mouse behavioral tests etc. -
Doctoral StudentLouisiana State University Jun 2014 - Aug 2019Baton Rouge, La, Us▸ Managed 3 projects that demand designing in vivo reporter (luciferase gene) assays and embryo injections that finally led to the development of an optimized transgenic animal model and 2 scientific publication.▸ Managed a team of 5 researchers in a project that ultimately led to the discovery of novel genes link that is involved in maintaining chromatin dynamics and 1 research article.▸ Participated and presented the findings in symposiums and conferences which helped in gathering new ideas to solve a problem that was persisting for 20 years in the lab.▸ Exceptional public speaking gained by instructing classroom of >180 undergraduate students for more than 3 years. Prepared UpToDate lecture slides and exams to guide students and to simplify complex genetics and molecular biology topics that was recognized by a prestigious departmental teaching award. ▸Efficient at risk management: learned from very effectively adapting to the situation when the major project needed to be changed for the successful completion of the project.Skills:PCR, qPCR, Reverse transcription PCR, immuno-affinity purification, co-immunoprecipitation (Co-IP), DNA/RNA isolation, Gel electrophoresis, EMSA/gel shift, Yeast 2 hybrid assays, cloning using Gibson assembly, general microscopy, SEM, embryo injections and generation of transgenic lines, classical genetics, and other relevant molecular biology and biochemical techniques. -
Graduate Research And Teaching AssistantLouisiana State University Jan 2013 - May 2014Baton Rouge, La, Us▸ Instructed and guided senior undergraduate students to understand and solve complex genetics related problems. Helped them individually and in the classroom to solve genetics related problem in practical ways. ▸ Mentored students individually and in the group, and actively engaged them to reinforce learning concepts and improve performance. Exercised active learning strategies in the classroom in order to provide the highest level of academic support to students and to understand basic concepts of biology.▸ As a lab instructor for freshmen level biology labs, prepared introductory presentations for students and supervised 6 hours of laboratory classes each week.▸ Actively mentored students to develop projects; help them to formulate and test the hypothesis to answer biological questions.
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Summer StudentCold Spring Harbor Laboratory Aug 2016 - Aug 2016Cold Spring Harbor, New York, UsI have participated in the summer course on Chromatin, Epigenetics, and Gene Expression, i.e. eukaryotic gene expression (GeneX).I got to learn hands on with state-of-art experimental technologies like Hi-C, ChIP-Seq, Chromatin remodelling assay, Luciferase assay, RNA expression and its informatics analyses.Besides the technical aspects, meeting the prominent scientists and experts inspired and taught me to seek more into this field and become a better scientist !!! -
Research And Development Associate ScientistNepal Agriculture Research Council Jan 2011 - Nov 2012Np▸ Efficiently planned and executed the study of 41 Rice varieties of different altitudes of Nepal by troubleshooting problems related to ELISA, primer design and PCR that resulted in the successful completion of the project ahead of the deadline.▸ Mentored 3 associates on the project, studied the marketplace and built connections with vendors that finally resulted in establishing a full functioning Molecular-Biology Lab in the Department.
Mukesh Maharjan, Ph.D. Skills
Mukesh Maharjan, Ph.D. Education Details
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Louisiana State UniversityBiochemistry And Molecular Biology -
Cold Spring Harbor LaboratoryGene Expression And Epigenetics
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Purbanchal UniversityBiotechnology
Frequently Asked Questions about Mukesh Maharjan, Ph.D.
What company does Mukesh Maharjan, Ph.D. work for?
Mukesh Maharjan, Ph.D. works for Takara Bio Usa, Inc.
What is Mukesh Maharjan, Ph.D.'s role at the current company?
Mukesh Maharjan, Ph.D.'s current role is Scientist.
What is Mukesh Maharjan, Ph.D.'s email address?
Mukesh Maharjan, Ph.D.'s email address is ma****@****ing.org
What schools did Mukesh Maharjan, Ph.D. attend?
Mukesh Maharjan, Ph.D. attended Louisiana State University, Cold Spring Harbor Laboratory, Purbanchal University.
What skills is Mukesh Maharjan, Ph.D. known for?
Mukesh Maharjan, Ph.D. has skills like Presentation Skills, Organoid Culture, Primary Immune Cell Culture, Cell Lines, Plasmid Construction, Flowjo, Elisa, Splinkerette Pcr, Western Blotting, Interpersonal Relationships, Immunoaffinity Precipitation, Teaching.
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