Muksheed Mohammad Shaik Email & Phone Number

Hyderabad, Telangana, India

Supporting New Product Introduction (NPI) project transfer and manufacturing scale up.▪ Implementing chemical and molecular biology test methods that have been developed by 3M labs.▪ Supporting Test Method validation and Transfer from internal or external labs.▪ Perform PCR and RT-PCR, ELISA, DNA & RNA Extraction.▪ Performing DNA/RNA detection using fully automated and semi-automated systems.▪ Submitting samples to sequencing.▪ Embeds specimens into blocks and fixes tissue in formalin.▪ Prepares solutions, stains, and reagents in accordance with standards written procedures.▪ Standardization of Concentrated antibodies for immunohistochemistry procedure.▪ Perform Immunohistochemistry and slide examination.

Stockholm, Sweden

involved in several projects to understand the genetic, cellular and environmental contribution towards inflammatory bowel disease (IBD) susceptibility, on other side I involved in one more project to discover the function of IBD-risk genes, by using animal models, including zebra fish and mouse, during this projects I did plenty of paraffin tissue sections, and complete immunohistochemisty staining along with slide examination.

Stockholm, Sweden

Hyderabad Area, India

Review of batch manufacturing records and batch packing records, Participated in regulatory audits and customer audits, Preparation of Master Manufacturing Formula (MMF), responsible for maintaining of training documents as per training SOP, Involved in preparationof Annual product quality review (APQR) and validation process, Reviewing and closing of deviations, Out of specifications, Change Controls, Incidents.

Uppsala, Sweden

Several genome-wide association studies have linked the Nudix hydrolase family member nucleoside diphosphate-linked moiety X motif 3 (NUDT3) to obesity. However, the manner of NUDT3 involvement in obesity is unknown, and NUDT3 expression, regulation, and signaling in the central nervous system has not been studied. We performed an extensive expression analysis in mice, as well as knocked down the Drosophila NUDT3 homolog Aps in the nervous system, to determine its effect on metabolism. Detailed in situ hybridization studies in the mouse brain revealed abundant Nudt3 mRNA and protein expression throughout the brain, including reward- and feeding-related regions of the hypothalamus and amygdala, whereas Nudt3 mRNA expression was significantly up-regulated in the hypothalamus and brainstem of food-deprived mice. Knocking down Aps in the Drosophila central nervous system, or a subset of median neurosecretory cells, known as the insulin-producing cells (IPCs), induces hyperinsulinemia-like phenotypes, including a decrease in circulating trehalose levels as well as significantly decreasing all carbohydrate levels under starvation conditions. Moreover, lowering Aps IPC expression leads to a decreased ability to recruit these lipids during starvation. Also, loss of neuronal Aps expression caused a starvation susceptibility phenotype while inducing hyperphagia. Finally, the loss of IPC Aps lowered the expression of Akh, Ilp6, and Ilp3, genes known to be inhibited by insulin signaling. These results point toward a role for this gene in the regulation of insulin signaling, which could explain the robust association with obesity in humans.

M.Sc Biomedicine, Biotechnology

Bachelor Of Pharmacy - Bpharm, Pharmaceutical Sciences

The objective of the study was to formulate Solid dispersions (SD) of Carbamazepine to improve the solubility and dissolution rate.

Intermediate, Zoology/Animal Biology, A