Matthew Talbot Email and Phone Number

Abbott Diagnostics Scarborough, Inc.• Committed initiator of continuous improvement through enaction of cross-functional Change Orders including training compliance, new standard operating procedures, and validation of equipment• Implemented training and process improvements related to inspection of lateral flow test strip control line positioning, resulting in projected annual savings of 97,000 USD and 11% improvement in agreement analysis between QC testing by DIGIVAL instruments and Production by Lanco Control Line Inspection Module; Lean Six Sigma project management• Identified validation gaps through review of equipment process validations (IQ, OQ, PQ, TMV, Cleaning Validation) for lateral flow reagent striping, test strip lamination, and automated device assembly• Served as a primary point of contact for Production compliance-related questions and responsible for overseeing conformance to ISO13485 and Title 21 CFR Part 820 Quality System Regulation• SME for Lateral Flow products• Equipment qualifications execution, analysis and report authorization• Evaluation and write-up of quality incidents (nonconforming materials, processes, or conditions), reprocessing records, SOPs and batch records

• Led a team of chemists in production, in-process evaluation, and product testing of BinaxNOW COVID-19 Ag lateral flow test chemical manufacture: nanoparticles, bioconjugation, final test strip sensitivity and specificity testing.• Addressed vendor material quality issues through cross-functional testing experimentation, leading to an optimized avidity and reagent concentration for immobilization. Supported introduction of new methods and materials for continual improvement of quality and efficiency.• Chemical Hygiene Officer responsible for compliance and safety in alignment with OSHA Laboratory Standard 29 CFR 1910.1450, and disposal decisions in accordance with local and federal regulatory bodies. Assisted in update of SOPs to include chemical disposal and safety information.• Biosafety Committee member- responsible for ensuring biological laboratory practices are up-to-date and in accordance with regulations.

• Led experimental design and execution in engineering of recombinant protein and cancer therapeutic. Assisted in writing grants to secure project funding.• Optimized a gene delivery assay for controlled release of AAV, transfection efficiency, and gene silencing• Experienced in mammalian and bacterial cultures: HEK293T, L929, HUVEC, H9 || BL21, DH5α• First-authored a book chapter summarizing research pioneering the use of biomimetic silk-elastinlike protein polymers for controlled release of anti-inflammatory drugs, viral gene therapy, and targeted imaging agents in the Royal Society of Chemistry's book, "Biomimetic Protein-based Elastomers: Emerging Materials for the Future."• Mentored undergraduates and PharmD students in research techniques and analysis• Fermentation and purification of recombinant protein polymers.• Designed and cloned new recombinant protein polymer genes.• Maintained bacterial cultures and mammalian cell cultures in accordance with GCCP.• Gel electrophoresis, PCR, ELISA, fluorescence microscopy, data acquisition and analysis• Evaluation of hydrogel-adenoviral HIF-1α shRNA for treatment of glioblastoma• Evaluation of recombinant protein polymers for improved cytoarchitecture and reproducibility of neural rosettes.Teaching Assistant for Molecular Biochemistry (Graduate Level)• Produced walkthroughs for students to evaluate and characterize a protein of interest utilizing online resources: PyMOL, Visual Molecular Dynamics, H++, HeliQuest• Held help sessions for protein characterization project• Assisted in writing and grading of the class final examination

1 of 3 incoming graduate researchers to be awarded this competitive fellowship which covered 1st semester tuition and stipend

Optimized bioprocessing and gene editing SOPs.Maintained electronic lab documentation and carried out SOPs and protocols for the fermentation and purification of silk-elastin like protein polymers.Further developed methods in upstream and downstream process control: genetic engineering and biosynthesis, cation and anion column chromatography, and tangential flow filtration.

Michael D. Mason LaboratorySupervised and instructed 2 undergraduate researchersAssisted in design and implementation of hardware, optical device elements, and 3-D printingDevelopment of flexible, conductive, and optically translucent nanocomposite thin filmsSynthesized gold nanoparticles, silver nanostars, nanowires, and nanoparticlesAssisted with development of various new procedures and testing methodsMaterial characterization utilizing Dynamic Light Scattering and UV-Vis SpectrophotometryCalibrated a rheometer for optical analysis of viscous fluidWork under Chemistry PhD candidate Muhammad Hossen, which included synthesis of monodisperse silver nanoparticles and a substrate for surface enhanced Raman spectroscopy, became the focus of my Honors thesis.Howell Biointerface and Biomimetics LaboratoryUMaine Internal Grant Co-AuthorBiomedical and industrial applications of micro-patterned paperMicrofluidic device design for life science and diagnostics, chemical deposition and functionalization of surfaces, flexible electronicsSupervisor for 2 undergraduates

Assisted with an undergraduate 400-level Bioengineering instrumentation course primarily focusing on programming in LabVIEWHelped students in design, construction, and programming of assignments, including that of a pulse oximeter

Facilitated an outreach project for Maine high schoolers at the University of Maine, "Consider Engineering," instructing students in synthesis and characterization of gold nanoparticles

Developed and tested a small integrated device optical sensorUtilized LabVIEW programming, Solidworks CADConstructed a proprietary light processing programPresented polished data including statistical analyses to the contacting team of engineers using Origin