Michelle Chan Email & Phone Number

Coquitlam, Bc, Ca

Vancouver, Canada

 Completed a honours thesis research with Dr. Sharon Gorski at BCGSC on the sensitisation of triple negative breast cancer cell line MDA-MB-321 towards conventional proteasome inhibitors (Bortezomib and MG132) and currently pursuing studies on the Autophagy level of Drosophila in parallel to my previous project.  Performed various subculturing and microscopic techniques on regular laboratory day basis to monitor and visualize cell growth Conducted Western Blot and Immunoblotting for protein identification Executed Proteasomal Activity Assay and siRNA knockdown to understand cellular proteasome function Performed Crystal Violet Assay to quantify the level of cell viabilities Optimized drug concentration for better characterization of protein levels

8888 University Dr, Burnaby, Bc V5A 1S6, Canada

 Prepared and characterized genomic DNA Performed plasmid miniprep and midiprep for DNA Extraction Carried out restriction enzyme digestion and amplified selective fragments using Polymerase Chain Reaction (PCR)  Ran Agarose Gel Electrophoresis and subcloned isolated DNA into bacterial plasmid Conducted site-directed mutagenesis to introduce mutation into DNA strains  Performed ELISA to investigate the antigen-antibody interaction

8888 University Dr, Burnaby, Bc V5A 1S6, Canada

 Screened P450cam mutants with polychlorinated substrates to examine their activities Conducted NADH consumption activity assays using UV-Vis Spectroscopy Performed bacterial transformation and agar plating to obtain co-transformed plasmids Carried out Ni-NTA column chromatography to purify proteins  Checked UV absorbance and performed SDS-PAGE to study proteins in crude lysate and dialysate Expressed and isolated P450cam mutants (with PdR and PdX) in E.coli in large scale

Honours Bachelor Of Science - (Hbsc), Biochemistry And Molecular Biology, Cgpa : 3.85

Activities and Societies:  Elected as the treasurer for the Malaysian and Singaporean Students Club at SFU to manage financial affairs.

American Degree Program (Adp), Biosciences, Cgpa : 3.72

Activities and Societies: ADP Student Congress