Nabil Rahman Email and Phone Number

Projects at the Fay Lab, Department of BiologyThermotolerance Assays on Hybrid strains● Assayed 120 hybrid strains of S.ceravisae and S.uvarum for thermotolerance dataOverexpression studies of CUP1 and SSU1 in yeast strains:● isolated the ura3-140 gene for transformation and cultured ura3- S.cerevisae cells● Transformed yeast strains with CUP1 and SSU1 alleles using gap repair cloning and high copy episomal vectors● Assayed cells using a microplate spectrophotometer (Biotek)Experimental evolution and genomic library preparation:● Extracted 576 mutated and ancestral yeast DNA from the wild● Prepared genomic libraries using Nextera library prep kits (ilumina) for extracted DNA, including primer design for PCR and barcoding for Next Generation Sequencing (NGS), followed by optimization of bioinformatics software(igv-viewer) to analyze sequencing data and identified variants in mutant and ancestral DNADesigned a corrective regression curve to compensate for yeast growth edge effects in 96, 384, and 1536 well plates:● Utilized genome screening robot and imager (ROTOR HDA and Phenobooth) to generate edge effect data● Designed a model and a corrective regression curve for data utilizing the R programming languageDomestication and Diversification of Yeast:● Extracted 25 wild yeast strain DNA by colony isolation, colony PCR, running electrophoresis gels, and prepared samples for DNA/Gene sequencing● Analyzed data and updated existing yeast genomic database

Tutored Part-time in the following courses:Intro to Biology II, Evolution, Biochemistry, and Genetics

Studied the role of microRNAs in the regulation of Acute Respiratory Distress Syndrome (ARDS)● Cultured mammalian cells and maintained Human Lung Epithelial (A549) and Human Bronchial Epithelium (16HBE) cell lines, using a hemocytometer to prepare cells for experimentation● Performed electroporation and transient transfections using β1 and MRCKα 3’UTR plasmids● Quantified gene expression using dual-Luciferase reporter assays

● Diagnosed and troubleshooted software/hardware issues on all platforms provided by customers through walk-in, chat and telephone facilities ● Assisted with network registration & troubleshooting for campus networks

Evaluated cancer remission probability using an algorithm that analyzes expression of oncogenes from FFPE breast tissue samples, lab duties included DNA/RNA extraction, RT- PCR, cDNA synthesis, etc.