Nick Afanasiev Email & Phone Number

San Diego, Ca, Us

San Diego, Ca, Us

Indianapolis, Indiana, Us

• Worked both independently and collaboratively as part of a preclinical-stage, new drug immunogenicity risk assessment group• Conducted cell-based laboratory experiments in accordance with written protocols and company’s standard operating procedures (SOPs)• Developed cell-based hTLR activation reporter assays• Maintained several different mammalian cell lines in culture• Wrote and revised experimental protocols• Analyzed and graphed experimental data using Microsoft Excel and GraphPad Prism• Presented results of experiments in weekly group meetings

Carlsbad, Ca, Us

• Worked in laboratory, vivarium, and office settings as part of a preclinical-stage, new neurologic drug discovery team• Followed established protocols to properly execute laboratory procedures, including performing tissue collection, RNA purification, and qPCR analyses• Handled live mice to measure body weights, check for signs of illnesses, etc.• Injected compounds into mice via multiple different routes of administration (SC and IV dosing)• Troubleshot experiments and found optimal solutions for routine problems• Analyzed experimental data and communicated results to team members

San Diego, California, Us

• Typically worked on multiple, GLP-regulated as well as non-GLP, preclinical-stage drug development projects in parallel• Performed in vitro experiments involving ELISA and cell-based assays• Cultured and banked mammalian cell lines using aseptic technique• Handled and dosed mice according to in vivo study design (SC, IP, IM, and PO dosing)• Monitored lab animals, including mice, rats, hamsters, pigs, dogs, and non-human primates (NHPs), for signs of adverse health conditions• Processed blood samples to plasma/serum• Utilized, maintained, and calibrated various automated lab equipment• Kept track of inventory and ordered laboratory supplies as needed• Visually inspected the laboratory procedures performed by others for quality control (QC) purposes

• Performed laboratory procedures, including running PCR, agarose gel electrophoresis, and molecular (gene) cloning, under the supervision of the principal investigator• Contributed to writing an article published in a reputable, peer-reviewed scientific research journal• Maintained accurate, detailed records of all laboratory procedures• Cultured both mammalian and bacterial cells• Isolated and purified DNA plasmids from bacteria• Transiently transfected mammalian cells• Dissected mice to collect tissues for downstream RNA analyses• Analyzed research findings and discussed results with supervisors

∙ Assisted in the instruction of four different college biology courses while learning how to teach under the mentorship of UC San Diego professors∙ Lead weekly class sections of students in which I reviewed course content presented by the course instructors during lectures∙ Taught supplementary material not covered by course instructor∙ Taught a weekly lab section together with one graduate student∙ Held weekly office hours, where students came to see me for help with their exam preparation and assignments∙ Supervised students while they worked on assigned experiments in lab∙ Graded coursework and exams∙ Helped improve exams by providing professors with feedback on exam drafts

Bala-Cynwyd, Pennsylvania, Us

• Engaged with customers as first point of contact both in-store and on the phone• Adhered to company’s customer care standards to meet customers’ expectations• Understood customers’ needs and desires and offered viable solutions to their problems• Sold automotive parts and accessories at a parts counter and cash register• Upsold products and services when warranted• Monitored inventory to ensure products remained in stock• Investigated and resolved customer complaints• Loaded and unloaded supplies from inventory delivery trucks on a weekly basis

• Worked collaboratively with varied groups of directors, other actors, and film crew members during filming of television commercials, TV show episodes, films, digital marketing campaigns, and a public service announcement (PSA)• Prepared for productions by analyzing scripts and memorizing lines verbatim• Performed as a principal actor in two different nationally-televised commercials (CCM Hockey and Mopar)• Took on day player roles in two episodes of TV shows (Animal Planet’s Killer Outbreaks and Nickelodeon’s iCarly)• Achieved a lead actor role in direct-to-video (and digital streaming platforms) feature-length film titled 2012: Ice Age• Enacted a principal actor role in rock band JP, Chrissie & The Fairground Boys’ official music video If You Let Me• Performed as a principal actor in Unilever’s global digital marketing campaign titled Cornetto Lick Challenge• Took on other lead, principal, and supporting actor roles

∙ Monitored company’s nationwide mobile medical examiners team for compliance with policies on exam status updates∙Provided friendly and effective customer service to company’s mobile medical examiners via telephone∙Built rapport and maintained positive professional relationships with company’s examiners∙Entered and updated data in computer software∙Sorted and filed records related to office activities∙Kept track of office supply inventory∙Performed other administrative duties as required