I showed the critical role of NK, mucosal ILCs and Monocytes in pathogenesis of SIV infection and during anti-retro-viral therapy (Virology. 2014 Feb;450-451:359-68, AIDS Res Hum Retroviruses. 2015 Jan;31(1):115-26)We replicated the RV144 HIV clinical trial in rhesus macaques with similar efficacy. In this study, I uncovered a correlation of mucosal IL-17 producing NKp44+ILCs with reduced risk of infection. This is the first ever report of association between mucosal NK/ILC and… Show more I showed the critical role of NK, mucosal ILCs and Monocytes in pathogenesis of SIV infection and during anti-retro-viral therapy (Virology. 2014 Feb;450-451:359-68, AIDS Res Hum Retroviruses. 2015 Jan;31(1):115-26)We replicated the RV144 HIV clinical trial in rhesus macaques with similar efficacy. In this study, I uncovered a correlation of mucosal IL-17 producing NKp44+ILCs with reduced risk of infection. This is the first ever report of association between mucosal NK/ILC and reduce rick of HIV/SIV acquisition (Nat Med. 2016 Jul;22(7):762-70). I also found a correlation between IFN-γ producing double negative (NKp44- NKG2A-) innate cells in mucosa with increased risk of SIV infection. We also found that the RAS signalling pathway was associated with protection SIV/HIV in RV144 like study in rhesus macaques (Nat Med. 2016 Jul;22(7):762-70). In collaboration with Dr. Rafick Sekaly's lab, I found the down-regulation of inflammatory genes in blood when ALVAC-SIV primed macaques were boosted with ALVAC-SIV and alum-adjuvanted SIV gp120 envelop protein.I tested the use of envelop specific passively transferred antibodies (passive immunization) together with vaccine recruited NK cells to mediate protection from SIV infection.Furthermore, I studied different vaccine vectors and adjuvants to develop an effective preventive vaccination strategy for HIV infection. Show less

Planned, designed, conducted, analyzed, and reported original research findings related to Respiratory syncytial virus (RSV) humoral responsesUse whole genome gene-fragment phage display libraries (GFPDL) and high throughput Surface Plasmon resonance (SPR) to identify RSV related humoral responses

Planned, designed, conducted, analyzed, and reported original research findings related to cytolethal distending toxin activity of Helicobacter species using cell lines, flow cytometry, purified recombinant proteins, nuclease assay, immunofluorescence microscopy, ELISA and western blot assays.Wrote manuscripts, abstracts and published 2 first author papers and 6 abstracts.Trained and supervised undergraduate students

Cloned and Identified thioredoxin bind protein-2 from human lens epithelial cells and pig eyes identified the role during the oxidative stress using in-vivo, in-vitro enzymatic, biochemical assays, mass spectrometry, western blot assays and immunofluorescence and electron microscopy. Wrote manuscripts, abstracts and published 1 first author papers and 3 abstracts