- Cell cultures.- Extraction and purification of plasmid and genomic DNA.- Extraction of RNA.- PCR and RT-PCR techniques.- Agarose gel electrophoresis.- Vector engineering for the expression of recombinant proteins in yeast.- Transformation of E. coli and yeast cells

Experience:- Supervisor of the purification stage of Biopharmaceutical processes on an industrial scale (Heberbiovac HB and Heberprot-p).- In the control, development and improvement of recombinant protein production processes on an industrial scale.- In compliance with Good Laboratory Practices.- In compliance with Good Manufacturing Practices.- In the validation of production processes.- In the statistical control of the process.- In stability studies of process mother solutions.- In the use of preventive control tools, such as risk analysis.- In the preparation and monitoring of corrective measures for non-conformities of the process.- In the development of standard production procedures.- As a supervisor of compliance with good documentation practices.- In the management of work groups to achieve quality indicators and production plans of the company.- In the preparation and training of personnel to perform in different stages of a Biotechnological process.- Skills in the management of programs such as Word, Excel and Power Point.

Experience- Tangential filtration with hollow fiber cartridges and filtration cassettes.- Determination of bubble point and diffusion speed for filter integrity testing.- Gel filtration chromatographic purification (conventional and by HPLC) on an industrial scale.- Scaling and de-scaling of chromatographic processes,- IFA sterilizing filtration with flat membranes and filter cartridges.

- Chromatographic purification by ion exchange, affinity for metals, affinity for protein A.- Spectrophotometry.- Hybridoma culture.- Development of diagnostic systems in bioline and ELISA format.- Conjugation of antibodies with colloidal gold.- Analytical techniques: Dot blot, Western blot, ELISA, SDS-PAGE, quantification of total proteins by the Bradford method.