Nancy Leal Reyes Email and Phone Number

Responsibility 1: In charge of the processes in the laboratory in cell culture of stem cells andtherapy cell• Responsible for the maintenance and cellular expantion of stem cells• In charge of transporting donation samples and expantion of umbilical cord tissue and itscryopreservation• In charge of the bone marrow sample maintenance and expantion procedure and itscryopreservation• Support in cell cryopreservation• Support sample collection in the clinical operation pavilion• Support the transfer of samples and therapies to and from the laboratory• Support laboratory quality control (microbiology)Responsibility 2: Assist in the performance of administrative laboratory procedures• Contribute to the execution of each of the laboratory projects, with high standards ofefficiency and quality.• Entry and backup of contracts and invoices• Management of payments and collections from patients• Make invoices and receipts from the SII• Sales income

Responsibility 1: Customer loyalty• Coordinate and execute the sales plan of the assigned portfolio• Visit scientific researchers from the Universities of the assigned portfolio• Support to the client in searching for the most appropriate reagent for their research: MolecularBiology, Cell Culture, Immunology, among others• Keep a record of visits made and those to be made• Make the quotes requested by the client from the CRM• Prepare sales notes, shipping guide and invoices from the CRM• Make invoices and receipts from the SII• Track the supplies or reagents that are ready to be shipped• Follow up of the quotes sent until the deal is closed.• Participation in National Congresses: Society of Cellular Biology of Chile (SBCCh-2022)Responsibility 2: Public Market• Direct work on the Public Market platform: Tenders and Agile Purchases• Be in contact with the client to be able to achieve the agile purchase award• Be in contact with the person in charge of the tender (Public Institution)Responsibility 3: Import Management• Manage import of the requested supplies.• Coordinate with the Warehouse the consolidation of the cargo to be imported.• Keep import documents and certificates of origin in order

Responsibility 1: COVID 19 Sample Processing, 4th shift• Processing of nasopharyngeal COVID19 samples from patients in extraction automatedequipment RNA (magnetic magnet).• Processing of nasopharyngeal COVID19 samples from patients manually, using a manualextraction kit by columna.• Performing qPCR, mounting the PCR plate and operating the qPCR thermal cycler equipment.• Analysis of the result and issuance of the exam.• Work experience in a level 2 biosafety cabinet.• Experience working in a PCR cabinet.• Experience working in a laboratory accredited by CAP.• Experience working under pressure in situations of high sample Flow.• Personal communication with the patient to clarify any doubts, either by telephone or in person.• BiosLIS software management

Responsibility 1: Research assistant in the area of cell signaling in neurobiology andneurodegenerative diseases• Responsible for the operation of the laboratory, at an organizational level and for training intechniques experimental projects for PhD students.• Coordination of scheduled experimental trials.• Training of PhD Thesis of Biochemistry, Biology and Chemistry-Pharmacy majors.• Performing primary culture of hippocampal neuron cells and mouse and rat embryo cortices asan expert level profesional. Maintenance and genotyping of mouse colonies transgenic.• Design of the dissection protocol and maintenance of the primary culture of neuron cellshippocampal and cortical cells of a single transgenic mouse embryo.• Implementation of strategies for the maintenance, growth and genotyping of 5 colonies oftransgenic mice, managing to improve productivity by 50%.• Co-author of different publications in international magazines

Responsibility 1: Primary culture of rat hippocampal neurons• Responsible for coordinating the execution of the primary culture of rat hippocampal neurons• Dissection and execution of the primary culture of rat hippocampal neurons• Maintenance and monitoring of the primary culture of rat hippocampal neuronsResponsibility 2: Assigned Experiments• Execution and performance of different experiments: IFF, PCR, Western Blot, among others