I am serving as an immune metabolism expert and have an extensive experience and knowledge in small molecule and T cells engager functional effects. I successfully modulated the tumor microenvironment (TME) in vitro for better immunotherapy outcomes.Achievements1. Discovered that Click molecule of bispecific engager named CD3-3HAE can accelerate T cells and PBMCs mediated tumor killing2. Developed two assays related to the immune-metabolism. I introduced the T Cell Metabolic Profiling and Cell Mito Stress Test3. Created an assay to monitor PBMCs cytotoxicity using bispecific CD3-3HAE T cell engager antibody in a real-time mannerLeading projects:a. Developed an assay to identify correlation between T cell fitness and response to Immunotherapy (Botensilimab)b. Managed T cell Isolation and Expansion and TCR transductionc. Guided Lab Team Members: I had the opportunity to instruct new members of the lab, in the technical facets and scientific principles of experiments. This was a distinctive experience, particularly as I trained scientists without a background in biology and immunology.d. Conducted small molecule screening using robotic system.

Project 1: Tumor microenvironment and exosome signaling in co morbid metabolic diseases. Molecular mechanisms of metastasis in breast and prostate cancer.Project 2: Immune exhaustion and PD-1/PDL-1 signaling in Tumor Infiltrating Lymphocytes (TILs) onhuman primary breast tumors in patients with comorbid metabolic diseases and type-2 diabetesProject 3: Novel plasma exosome biomarkers for prostate cancer progression in co-morbid metabolic disease.● Supervised and instructed graduate and medical students in conducting and analyzing research projects.● Developed protocols of microRNA PCR array, TIRF microscopy, Ingenuity Pathway Analysis (IPA), exosome characterization, TCGA and Kaplan-Meier analysis, gene overexpression and adipocyte differentiation.● Developed ImageJ cell morphology analysis, exosome RNA labeling and delivery to cell nuclei, confocal microscopy and flow cytometry.

Research Project #1: Molecular mechanisms of breast cancer metastasisResearch Project #2: Effect of CRISPR-Cas9 Mediated NOX4 Knockout on invasion and focal adhesion formationResearch Project #3: Role of PIPKIγ90 phosphorylation on cell migration and invasionResearch Project #4: Role of sphingolipid metabolism in colorectal cancer metastasis.● Set up protocols of western blotting, CRISPR knockdown, invasion, migration, focal adhesion, invadopodium and PCR (TaqMan, Real Time).● Conducted molecular cloning, mutagenesis protein binding and Immunoprecipitation, ubiquitination and degradation

Role of PIPKIγ90 and NOX4 in cancer cell invasion and migration

Research Project #1: Profiling microRNA processing enzymes Drosha and Dicer in blood lymphocytes of patientswith gestational diabetes mellitus and multiple sclerosisResearch Project #2: Anti-cancer effects of Curcumin analogues on gastric adenocarcinoma and esophageal squamouscell carcinoma cells● Led the clinical research projects in collaboration with gynecology section● Managed the data acquisition and analysis in the cardiac surgery department● Wrote manuscripts of the basic and clinical research projects● Taught full courses in the medical school: Biochemistry (Nursing, Anesthesiology and Public Health) Students