Nathan Cawte Email & Phone Number

In addition to the duties of a lab technologist, my job includes tasks in data analysis, quality control + assurance, project management, and research + development. -Expanded on a custom methylation array to increase regions cover by 10x, and enabled processing of 3 large academic cohorts (~25,000 individuals). -Designed automated robotic liquid handling procedures using "Opentron" to automate 4+ laboratory processes, including DNA normalization of custom volumes. -Implemented project management principles to effectively manage over 100 tasks using ClickUps software. -Created quality control, and analysis pipelines for multiple methylation cohort studies using R Coding.-Completed an investigation that identified and rectified an operational issues effecting quality control of laboratory specimen processing. -Directly processed 300+ RNA expression samples and 1500+ samples on a custom genotyping array using molecular biology techniques.

Canada

https://age-rate.com/en-ca

Canada

Accomplishments include:-Successfully created and launched a direct to consumer blood test that reveals a persons biological age and non-medical health insights via epigenetic analysis. The test is combined with a mobile app to deliver lifestyle recommendations to users.-Created a custom Illumina Infinium HD methylation array, to predict biological age and 20 additional health insights at a 5 fold reduced cost compared to the gold standard method. -Achieved a $50,000 "Mitacs Accelerate Grant" by executing a whole genome bisulfite sequencing experiment and evaluating it's utility to predict biological age. -Bioinformatically designed 2 custom next generation sequencing panels that measured 140+ methylation sites at 100x coverage. -Created a novel method of DNA methylation quantification using methylation specific primers and microfluidic qPCR, measuring 42 methylation sites with an R2 of 0.96. -Obtained and formatted publicly accessible datasets of 14,000 + individuals with DNA Methylation and phenotypic data. -Created over 50+ scripts in R for DNA methylation quality control and analysis, including multiple machine learning algorithms that predict age with an R2 of 0.91 and RSE of ~4 years. -Explored FDA and Health Canada medical device regulations to design the AgeRate blood collection method. -Created a method of stabilizing DNA methylation for 30+ days at various temperatures by utilizing a unique buffer. -Made SOPs, tracking sheets, protocols, quality metrics and more to establish laboratory operations. -Fully automated company operations from fulfillment to results upload, with the ability to process 24-384 samples a week, in the US and Canada.-Grew company to 250+ users, and $350,000+ investment raised.

Hamilton, Ontario, Canada

-Developed a Custom Illumina Infinium HD Methylation Array run on over 5000 samples, a total project cost of ~$400,000. This serviced 2 large academic projects and 1 commercial interest. -Designed and tested a low-cost duplex qPCR assay to measure mitochondrial copy numbers in a study of more than 10,000 individuals. Resulted in 2 high impact publications. -Created and validated an assay to measure haptoglobin genotypes with qPCR, ran in over 7000 individuals.-Trained and supervised 3 students conducting bioinformatic projects during their undergrad research course. Each scoring highly on their work (A- to A+).-Automated robotic processing on the Agilent Bravo System to automatically plate telomere qPCR assay, and processed on ~700 samples. -Organized run templates, protocols, and SOPs for several routine laboratory processes.-Processed over 200 exome samples on Ion Torrent Sequencing. -Performed a 1 year CO-OP during the timeframe of 2016-2017 at the lab, maintained good relationship and hired into this position after graduation.

Hamilton

- Led a team of 5 individuals to successfully recruitment, perform sample distribution, sample collection, and initial physical scans of 500 participants in GeneBlueprints DNAble clinical trial. - Created a custom nutrition mapping tool to suggest optimal macronutrient ratios, calorie intakes and commercial diets optimized for an individual’s genetics. - Designed a 150-page booklet explaining in detail the GeneBlueprint's product for resellers.- Made 30 social media posts to successfully increase GeneBlueprints popularity and sales. - Created 10 genetic tables aimed to tell consumers about their unique genetic profile, showing the genetic trait, population frequency, the effect of ethnicity, and gene function. - Create a tool for identifying over 50 potential new products to meet the stringent quality of GeneBlueprints products. - Performed market research to understand the competitive landscape for genetic testing products.

Hamilton, Ontario, Canada

- Designed and performed telomere analysis of over 500 participants using qPCR technology, all achieving gold standard results. - Performed genotyping on the genome wide screening array with over 50 samples. - Worked directly with suppliers to ensure adequate materials for 3 projects. -Optimized DNA extraction protocols to achieve accurate and cost effective results for commercial genotyping. - Participated in a genotyping project aimed to test and analyze alleles of participants with the FTO gene- Experienced with gene sequencing exome regions and targeted regions with the ion torrent X5, and pre sequencing steps including DNA quantification, sequencing preparation and qPCR.- Qualification and quantification of DNA using picogreen assay, Qubit assay, real time PCR, bioanalyzer and fragment analyser.- Knowledgeable in Illumina IScan genotyping protocol and preparation steps.- Assisted in general laboratory tasks including maintenance of equipment and lab space.- very experienced in applying PCR to analyze specific gene mutations and regions.- Assisted in research for over 5 ongoing projects in the genetic and molecular epidemiology lab.- Helped with administration tasks including storage, inventory and data maintenance. - Ensured Safe workplace practices by updating over 100 biological and chemical MSDS forms- Very experienced with qPCR applications like genotyping and quantification using the ion torrent Via VII system.-Used gene sequencing and genotyping machine that cost $200,000+ without a flaw demonstrating my ability to work with complex machinery and costly assay protocols.

Hamilton, On

Ontario, Canada

worked summers starting in 2012•Responsible for the safe and well been of others in the facility.•Left me physically and mentally in shape for many different situations, and different environments.•Have learned how to be responsible and to have a good work ethic at all times.•First aid certifications and experience.

worked summers starting in 2012•Responsible for communication and safe toward children, parents and adults.•Formed great communication skills, teaching skills, and social skills.•Complete and submit progress report cards for up to 60 people a session.•Created lesson plans suitable to a certain age/experience level that will best tend to their needs, and help improve their technique.•Other life skills acquired are leadership, professionalism, respect and confidence.

Milton, Ontario, Canada

Provided technical expertise by:-Conducting orientation and in-service training of staff to ensure an applied understanding of life support programs (emergency procedures) and techniques, lesson instruction, and knowledge of departmental policies and procedures;-instructing advanced level aquatic instructor and lifeguarding programs acting as a mentor for staff ensuring established safety and customer service standards are met; -providing input, organizing & supervision -made sure the facility was safe to open and close by completing a check listProvided supervision by:- planning, assigning, supervising, and checking the work of employees engaged in instructional and lifeguarding duties;.- participating in the training, and development process for staff;- evaluating the work performance of Aquatics staff;Provided customer service by:-communicating effectively with staff (aquatic, administration, maintenance) and public, fostering a safe and enjoyable facility;- answering enquiries and handling complaints of participants;- establishing and maintaining effective working relationships with a variety of internal and external contacts.Provided administrative duties by:-used a program registration, data input and organizational computer program to perform tasks-preformed related duties as assigned

worked part time until September 2014, return to work for the summer after school.•Responsible for the safe and well been of others in the facility.•Left me physically and mentally in shape for many different situations, and different environments.•Have learned how to be responsible and to have a good work ethic at all times.•First aid certifications and experience.

worked part time until September 2014, return to work for the summer after school.•Responsible for communication and safe toward children, parents and adults.•Formed great communication skills, teaching skills, and social skills.•Complete and submit progress report cards for up to 60 people a session.•Created lesson plans suitable to a certain age/experience level that will best tend to their needs, and help improve their technique.•Other life skills acquired are leadership, professionalism, respect and confidence.

Bachelor'S Of Technology (B. Tech), Biotechnology, 3.7/4

Graduate of an integrated 4.5 year joint program with McMaster University and Mohawk College. -Graduated with "Summa Cum Laude".

Advanced Diploma, Biotechnology, 3.7/4

Certificate, Business Management, 3.7/4