Hands-on experience with a broad range of biochemical, cell-biological, immunological, microbiological, virological, and molecular-biological techniques including: - Biolayer interferometry (BLI, Sartorius, Octet) for screening purposes and to study antibody-antigen interactions (binding, competition, and affinity measurements).- Surface plasmon resonance (SPR, Cytiva, Biacore) for screening purposes and to study antibody-antigen interactions (binding, competition, and affinity measurements).- Flow cytometry (BD Biosciences FACS Canto II and other equipment) to analyze antibody-dependent cell-mediated cytotoxicity, complement-dependent cytotoxicity, interactions between antibodies and surface components of bacterial and mammalian cells and to detect proteins inside mammalian cells.- All kinds of enzyme-linked immunosorbent assay (ELISA) formats (design, development & qualification), radioimmunoassays & (immuno)cytology.- Bacterial diagnostics.- One-dimensional polyacrylamide gel electrophoresis of proteins & western blotting. -Mammalian cell culture & cell transfection, infection, and transduction (for protein production, adenovirus production or transgene expression).-Protein production, (affinity) purification, labeling, quantification and characterization (using various methods).- Adenovirus batch production (including plaque purification) and (CsCL) purification for research, HDEP and preGMP purposes including release assays, CofA, study plan and study report.- Virological assays: OD260, transgene expression & westernblot, VP-qPCR, QPA, ID PCR (identity and integrity), sanger sequencing results analysis (identity), productivity assay, reduced scale model productivity assay (Presence), transgene stability assay.- Molecular cloning: pUC to pAd by restriction enzyme digests & IVA cloning, bacterial transformation, mini & maxi culture & preps, restriction enzyme analysis, sanger sequence results analysis- Polymerase chain reactions (e.g. random amplification of polymorphic DNA, diagnostic, identity and integrity) & restriction fragment length polymorphism analysis- DNA agarose gel electrophoresis (including pulse-field gel electrophoresis) & Southern blotting.
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Associate ScientistJanssen Inc. Aug 2000 - Nov 2024Leiden, South Holland, NetherlandsBiochemical, cell-biological, immunological, microbiological, virological, and molecular-biological techniques: - Biolayer interferometry (BLI, Octet) and Surface plasmon resonance (SPR, Biacore) for screening purposes and to study antibody-antigen interactions (binding, competition, and affinity measurements).- Flow cytometry (BD FACS Canto II and other equipment) to analyze antibody-dependent cell-mediated cytotoxicity, complement-dependent cytotoxicity, interactions between antibodies and surface components of bacterial and mammalian cells and to detect proteins inside mammalian cells.- All kinds of enzyme-linked immunosorbent assay (ELISA) formats (design, development & qualification).- One-dimensional polyacrylamide gel electrophoresis of proteins & western blotting.- Mammalian cell culture & cell transfection, infection, and transduction (for protein production, adenovirus production or transgene expression).- Protein production, (affinity) purification, labeling, quantification and characterization (using various methods).- Adenovirus batch production (including plaque purification) and (CsCL) purification for research, HDEP and preGMP purposes including release assays, CofA, study plan and study report.- Virological assays: OD260, transgene expression & westernblot, VP-qPCR, QPA, ID PCR (identity and integrity), sanger sequencing results analysis (identity), productivity assay, reduced scale model productivity assay (Presence), transgene stability assay.- Molecular cloning: pUC to pAd by restriction enzyme digests & IVA cloning, bacterial transformation, mini & maxi culture & preps, restriction enzyme analysis, sanger sequence results analysis. -
Research TechnicianDepartment Of Clinical Immunology, University Medical Center Groningen Jul 1998 - Jul 2000Groningen, NetherlandsWork experience in the context of research into the role of T cells activation by S. aureus Superantigens and acid phosphatase (SAcP) at the Wegener's disease (WG).Biochemical, cell-biological, immunological, microbiological and molecular-biological techniques
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Medical Microbiological Research TechnicianDepartment Of Medical Microbiology, University Medical Center Groningen Apr 1991 - Jun 1998Groningen, NetherlandsBacteriological diagnostics & Immunological techniques. Molecular biological techniques for epidemiological research into hospital bacteria such as MRSA.
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Microbiological TechnicianAnimal Health Service - Drachten Jul 1983 - Mar 1991Drachten, Friesland, NetherlandsBacteriological and mycological diagnostics, histological and immunological techniques.
Nelie Van Dijk Education Details
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Center For Biomedical Technology, Faculty Of Medical Sciences, University Of GroningenCertificate -
Horizon Training & DevelopmentCertificate -
Senior Secondary Vocational Education At The Stichting Analisten Opleiding FrieslandDegree -
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Frequently Asked Questions about Nelie Van Dijk
What is Nelie Van Dijk's role at the current company?
Nelie Van Dijk's current role is Associate Scientist, creative en resultaatgerichte professional die bezig is met een carrière switch naar grafisch vormgever..
What schools did Nelie Van Dijk attend?
Nelie Van Dijk attended Center For Biomedical Technology, Faculty Of Medical Sciences, University Of Groningen, Horizon Training & Development, Senior Secondary Vocational Education At The Stichting Analisten Opleiding Friesland, Hogeschool Leiden, Hogeschool Leiden, Hogeschool Leiden, Hogeschool Leiden.
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