Nelson Wu Email & Phone Number

Cambridge, Massachusetts, United States

Combine computational modeling and yeast display to engineer malaria vaccine immunogen candidates for experimental validation and in vivo evaluationConstruct a mutation scoring benchmark to evaluate elicited HIV-1 antibodies from clinical trials

Isolated and characterized broadly neutralizing antibodies using gene analysis, ELISA, and Luminex bead-based assaysUtilized PCR, site-directed mutagenesis, and plasmid preparation to create recombinant antibody and HIV-1 envelope clones for transient transfections and purification using aseptic techniquesSet up a system for fluorescence-activated cell.

Received, accessioned, and set up patients’ bloods for 72 hour T-cell stimulation in peripheral blood cultures in accordance with patient filesPurified and harvested blood cultures during mitotic arrest, using hypotonic and fixative treatment in a six hour processFixed cultures to slides for Giemsa staining and performed constitutional karyotypes analysis

Analyzed antagonists’ effects on alpha-synuclein aggregation by Thioflavin-T binding assay for Parkinson's Disease studiesUsed filter-retardation assays and Western blots to determine aggregates’ and oligomers’ SDS-stabilityInvestigated secondary structure of aggregates with Fourier Transform Infrared Spectroscopy and Transmission Electron Microscopy

Guide students in general chemistry lab fundamentalsHold office hours for 6+ hours a weekTeach groups of 20-30 students at a time

Doctor Of Philosophy - Phd

Bachelor Of Science (B.S.), Biomedical Engineering

Activities and Societies: Chinese Association Choreographer, Biomedical Engineering Society