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Assay Developer with 5+ years of experience in developing and validating highly sensitive and specific assays to detect and measure synthetic biomarkers, ADA, NAb, DC-Tcell activation in biological samples (serum, PBMC, tissue lysates,...) for clinical sample analysis. Developed and validated 10+ PK/PD and immunogenicity assays, including ELISAs, MSD & Luminex assays, and cell-based assays. Proficient in using GraphPad Prism, Python, and FlowJo software for data analysis and presentation. Passionate about developing innovative assays to support the development of safe and effective biologic therapeutics.In addition, I have 8+years in designing, fabricating, and testing microfluidic devices for multiple applications (tumor on chips, diagnostics, and nanopore sequencing)
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Senior ScientistEarli Inc.Fremont, Ca, Us -
Senior ScientistPrognomiq Inc Feb 2024 - PresentSan Mateo, California, Us -
Senior ScientistEarli Inc. Sep 2022 - Jul 2024Redwood City, California, Us● Developed and validated customized PK/PD assays for preclinical and clinical targets in tissues and plasma/serum using MSD, Luminex, and Ella platforms.● Led the development and qualification of protocols to assess the immunogenicity of clinical targets, including clinical chemistry/CBC, histology, cytokines, ADA, T-cell activation, and plasmid integration, for Investigational New Drug submission.● Performed scale-up recombinant protein production, purification, and quality control using nickel column, AKTA FPLC explorer, and LCMS.● Developed functional cell-based assays to assess the therapeutic efficacy of synthetic therapeutic markers.● Performed assay qualification and wrote reports for transferring and training other personnel.● Led the analysis of clinical samples for efficacy and immunotoxicity in GLP-like settings.● Drafted clinical SOPs and wrote reports for the human clinical trial.● Managed and coordinated sample shipment, storage, inventory, and expense for the clinical trial.● Manage CROs for preclinical samples analysis (immunohistochemistry, long-read sequencing, capillary Western blot,…)
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ScientistEarli Inc. May 2021 - Sep 2022Redwood City, California, Us
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Visiting ScientistLawrence Livermore National Laboratory May 2018 - May 2021Livermore, Ca, Us● Discover the fusogenic ability of carbon nanotubes porin (CNTP) and investigate its mechanism● Developed novel CNTP lipid nanoparticles (cationic and ionizable lipids) encapsulated with RNA/DNA to improve the transfection efficiency of human breast cancer cells, human mesenchymal stem cells, and human dendritic cells using luciferase/EGFP SAM-mRNA, FACS, and microscopy ● Developed protocol for capped, poly-A tail RNA (1000-10000 bp) synthesis and quality control using cloning, RNA transcription, bioanalyzer, and lipofectamine assay● Developed a novel drug delivery system using liposomes embedded with CNTP for target-delivery of doxorubicin (DOX) to neuroblastoma and glioma cells and human breast cancer cells at much lower encapsulated concentration of DOX (10 µg/ml) compared to commercial Doxil (2mg/ml) with the same cell viability (~10%)● Designed and conducted the animal study to understand the cytotoxicity and the biodistribution of CNTP, and efficiency of CNTP-facilitated drug delivery using doxorubicin on MDA-MB-231 xenograft tumor models ● Studied the CNTP-induced differentiation of human mesenchymal stem cells into osteoblast, adipocyte, neural cells, and chondrocyte by looking at immunohistochemistry using antibodies and dyes as well as cell morphology -
Postdoctoral ResearcherUc Merced May 2018 - May 2021Merced, California, Us -
ScientistUniversal Sequencing Technology Jun 2016 - May 2018Canton, Ma, Us● Designed, developed, troubleshot, and optimized a novel microfluidic chip for testing the transport of single molecules across biological/synthetic nanopores on different materials (SiO2, Si3N4, Teflon, PEEK) and nano/microfabrication● Fabricated solid state nanopore using thin film deposition, photolithography, wet/dry etch, and TEM.● Developed protocols to modify and immobilize DNA on glass and silicon surfaces using different linkers● Develop a micromagnet chip to pull DNA through the nanopore at nm step intervals. -
Research AssistantBoston University Aug 2010 - May 2016Boston, Ma, Us● Designed microfluidic chips using CAD and soft lithography to quantify antimalarials using standard curves on chips, achieving >5% error compared to gold-standard methods (HPLC-UV and LC-MS)● Built, evaluated, and troubleshot the performance of the prototype from scratch to ready platform for field-testing in 4 months, achieving over 15% improvement in accuracy compared to GPHF-MiniLab system developed by Merck● Developed a Systematic Evolution of Ligands by Exponential Enrichment (SELEX) protocol to discover DNA aptamers bound to antibiotics using gel electrophoresis, bacterial cloning, next generation sequencing (NGS), qRT-PCR● Developed and optimized luminescent assays for detection of Artesunate (ATS) and Amodiaquine (AQ) with high accuracy, precision, selectivity, specificity, and sensitivity.● Designed field tests for Accra, Ghana and analyzed results to provide promising data with high accuracy● Designed, fabricated, characterized, and validated low cost and disposable microfluidic chipsusing cutter plotter, hot embossing and injection mold for concentrating human plasma by a factor of 8 by volume● Prototyped, developed, and troubleshot the performance of the RNA/DNA amplification chip with human plasma/blood samples spiked with Ebola-modified VSV, HIV, and Hep. B viruses using multiplex RT-PCR and RNA in BSL-2.● Integrated the microfluidic chip with an Interferometric Reflectance Imaging Sensor to form a diagnostic platform in close fashion to detect as little as 200 viral particles/ml with only 1 ml of human plasma
Nga Ho Skills
Nga Ho Education Details
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Boston UniversityBiomedical/Medical Engineering -
University Of California, BerkeleyBioengineering And Biomedical Engineering
Frequently Asked Questions about Nga Ho
What company does Nga Ho work for?
Nga Ho works for Earli Inc.
What is Nga Ho's role at the current company?
Nga Ho's current role is Senior Scientist.
What is Nga Ho's email address?
Nga Ho's email address is nho2311@bu.edu
What is Nga Ho's direct phone number?
Nga Ho's direct phone number is +185727*****
What schools did Nga Ho attend?
Nga Ho attended Boston University, University Of California, Berkeley.
What skills is Nga Ho known for?
Nga Ho has skills like Cell Culture, Fluorescence Microscopy, Matlab, Biomedical Engineering, Hplc, Ngs, Autocad, Soft Lithography, Comsol, Microfluidics, Fmea, Gel Electrophoresis.
Who are Nga Ho's colleagues?
Nga Ho's colleagues are Sathyapriya Rajagopal, Vy Nguyen, Henry Lee, Mohammed Goryawala, Xiao Bin Wu, Clara Lemus, Blaine Mccarthy.
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