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Ngoc Lam is listed as Biomedical Scientist/Cell and Molecular Biologist in Disease Research based in Greater Milwaukee, United States. AeroLeads shows a matched LinkedIn profile for Ngoc Lam.
Ngoc Lam previously worked as Research Scientist at Medical College Of Wisconsin and Assistant Researcher at University Of California, Santa Cruz. Ngoc Lam holds Ph.D., Cell/Cellular And Molecular Biology from University Of Arkansas At Fayetteville.
Scientist in the fields of molecular biology, cardiovascular disease and stem cell research. Highly experienced in mammalian cell culture, stem cell culture, drug testing and animal work. Led multiple interdisciplinary projects to study heart cell/stem cell pathophysiological behaviors and cell signaling as well as generate biomaterials and in vitro/in vivo disease models. An excellent collaborator, writer, and communicator dedicated to advancing treatments for disease. Core competencies: - Primary cell isolation and culture- Stem cell maintenance and differentiation- 2D and 3D in vitro cell models- In vivo mouse models- Molecular Biology - Communication, management
Listed skills include Research, Molecular And Cellular Biology, Mammalian Cell Culture, Cell Culture, and 37 others.
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Working on in vitro and in vivo models to identify therapeutic targets for the treatment of cardiomyopathy. In vitro model involves the use of induced pluripotent stem cell-derived cardiomyocytes. In vivo model utilizes mdx mouse model.
Santa Cruz, California
Worked in a bacterial pathogenesis and drug discovery lab. The work involved plasmid transfection, cloning, protein expression and purification, and animal (mouse) work
Fayetteville, Arkansas
Multiscale investigation of the behavior of heart valve interstitial cells in response to pathological shape and mechanical stimulationProject 1: Developed single cell model and tailored cell shape to study the inter-relationship between pathological cell structure and cell functionTechniques used: Primary cell culture|| Photolithography||Microcontact printing||Immunofluorescence|| Traction force microscopy||Two-photon redox imagingPublication: Lam, N. T., Muldoon, T. J., Quinn, K. P., Rajaram, N., & Balachandran, K. (2016). Valve interstitial cell contractile strength and metabolic state are dependent on its shape. Integrative Biology, 8(10), 1079-1089.Project 2: Developed collagen-based hydrogel for 3D culturing of heart valve interstitial cells in the presence of uniaxial cyclic stretchTechniques used: Scanning electron microscopy || Swelling and degradation studies || MTT assay || Cell viability/Cytotoxicity assay || Gelatin zymography || SDS-PAGE || Western blot ||Publication: Lam, N. T., Lam, H., Sturdivant, N. M., & Balachandran, K. (2017). Fabrication of a matrigel–collagen semi-interpenetrating scaffold for use in dynamic valve interstitial cell culture. Biomedical Materials, 12(4), 045013.Project 3: Investigated the stretch-mediated signaling pathway in heart valve interstitial cells using 3D modelTechniques used: Immunohistochemistry||Drug dose determination studies || Pharmacological inhibition|| Western blot || qRT-PCR || Immunofluorescent staining|| Two-photon redox imagingPublication: The role of FGF1 and FGF2 on the pathological behavior of valve interstitial cells in a three-dimensional mechanically-conditioned model (In print).
Fayetteville, Arkansas
Stem cell maintenance and differentiation Cultured induced pluripotent stem cells and human embryonic stem cells Optimized and used modified cell culture media to differentiate them into insulin-producing cells
Isolation of endothelial progenitor cells derived from human umbilical cord blood (Undergraduate thesis 2009-2010) Directly collected human blood from the umbilical cord Isolated mononuclear cells from blood sample Cultured and characterized endothelial progenitor cells
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Ngoc Lam is listed as Biomedical Scientist/Cell and Molecular Biologist in Disease Research.
Ngoc Lam is based in Greater Milwaukee, United States.
Ngoc Lam has worked for Medical College Of Wisconsin, University Of California, Santa Cruz, University Of Arkansas, and University Of Agriculture And Forestry, Ho Chi Minh City, Vietnam.
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Ngoc Lam holds Ph.D., Cell/Cellular And Molecular Biology from University Of Arkansas At Fayetteville.
Ngoc Lam is listed with skills including Research, Molecular And Cellular Biology, Mammalian Cell Culture, Cell Culture, Molecular Biology, Pcr, Microsoft Office, and Data Analysis.
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