• Engineered CRISPR-guided artificial transcription factors (aTFs) for epigenome editing• Explored novel applications of aTFs for manipulating cellular phenotypes• Collaborated with Groupd in MGH Cancer Center for applying aTFs in therapeutic context• Completed the MGH Cancer Center's 12-week Introduction to Bioinformatics Course (May 2019)

• Built custom quantitative PCR assays for real-time assessment of cellular methylation state• Developed cell-based assays and utilized flow cytometry to characterize CRISPR-Cas9 edited T-cells• Built method for preparing T-cell receptor DNA libraries and analyzed T-cell receptor diversity via NGS• Assessed cellular methylation state via the development of a bisulfite sequencing method and analysis pipeline

• Express and purify proteins for structural analysis via size exclusion, ion exchange, and affinity chromatography• Perform assays to establish Michaelis-Menten kinetics of purified enzymes• Develop binding assays to evaluate small molecule inhibitors of cannabinoid pathway enzymes

• Designed and implemented complex amplification schemes to create a bias-limited antibody DNA repertoire• Produced antibody libraries against peptide targets by utilizing yeast surface display technology• Identified candidate antibodies via enrichment for, panning of, and screening of library antibodies through magnetic bead based and flow cytometric methods• Collaborated with colleagues in a team oriented environment through frequent discussion of strategy/methodology and… Show more • Designed and implemented complex amplification schemes to create a bias-limited antibody DNA repertoire• Produced antibody libraries against peptide targets by utilizing yeast surface display technology• Identified candidate antibodies via enrichment for, panning of, and screening of library antibodies through magnetic bead based and flow cytometric methods• Collaborated with colleagues in a team oriented environment through frequent discussion of strategy/methodology and presentation of collected data Show less

• Validate optimal methods for the culture of iPSC-derived neurons and astrocytes• Characterize cell phenotype through qRT-PCR, immunocytochemistry and lipidomics• Develop procedures for substrate reduction therapy assays• Analyze, reproduce, and recapitulate information from primary source literature

•Referee intramural sports games and setup equipment

•Assist customers, order and receive inventory, reconcile cash and open/close store