Nicholas Perry Email & Phone Number

Palo Alto, CA, US

• Engineered CRISPR-guided artificial transcription factors (aTFs) for epigenome editing
• Explored novel applications of aTFs for manipulating cellular phenotypes
• Collaborated with Groupd in MGH Cancer Center for applying aTFs in therapeutic context
• Completed the MGH Cancer Center's 12-week Introduction to Bioinformatics Course (May 2019)

Cambridge, Massachusetts, USA

• Built custom quantitative PCR assays for real-time assessment of cellular methylation state
• Developed cell-based assays and utilized flow cytometry to characterize CRISPR-Cas9 edited T-cells
• Built method for preparing T-cell receptor DNA libraries and analyzed T-cell receptor diversity via NGS
• Assessed cellular methylation state via the development of a bisulfite sequencing method and analysis pipeline

Northeastern University

• Express and purify proteins for structural analysis via size exclusion, ion exchange, and affinity chromatography
• Perform assays to establish Michaelis-Menten kinetics of purified enzymes
• Develop binding assays to evaluate small molecule inhibitors of cannabinoid pathway enzymes

Cambridge, Massachusetts

• Designed and implemented complex amplification schemes to create a bias-limited antibody DNA repertoire
• Produced antibody libraries against peptide targets by utilizing yeast surface display technology
• Identified candidate antibodies via enrichment for, panning of, and screening of library antibodies through magnetic bead based and flow cytometric methods
• Collaborated with colleagues in a team oriented environment through frequent discussion of strategy/methodology and… Show more
• Collaborated with colleagues in a team oriented environment through frequent discussion of strategy/methodology and presentation of collected data Show less

Waltham, MA

• Validate optimal methods for the culture of iPSC-derived neurons and astrocytes
• Characterize cell phenotype through qRT-PCR, immunocytochemistry and lipidomics
• Develop procedures for substrate reduction therapy assays
• Analyze, reproduce, and recapitulate information from primary source literature

Northeastern University

• Referee intramural sports games and setup equipment

Marblehead, MA

• Assist customers, order and receive inventory, reconcile cash and open/close store

Bachelor Of Science - Bs, Summa Cum Laude, Biochemistry

• Dean’s List—All Academic Semesters • Dean’s Scholarship Recipient • Forbes Under 30 Scholar, 2016 • Northeastern University Honors.

High School Diploma, Salutatorian