Nicholas Lien Email & Phone Number

San Jose, California, United States

• Daily management of FLIPR product production while maintaining product quality, on time delivery, inventory, and productivity.
• Operating High-Performance Liquid Chromatography (HPLC) device to check product quality.
• Generate and submit Engineering Change Orders (ECOs) as needed for manufacturing related changes.

San Jose, California, United States

• Developed RNA-seq pipeline using Linux, Python, and R tools to process RNA-seq data and analyze gene expression information to study how microgravity may affect embryonic gene expression.
• Identified top 10 genes of interest by quantifying differences in gene expression between sample groups.
• Utilized standard bioinformatics tools and databases (GenBank, BLAST, DESeq2, STAR mapping, etc)
• Discovered that microgravity may affect the development of zebrafish embryos by upregulating genes that control cell proliferation, differentiation and development.

San Jose, California, United States

• Utilized bioinformatic approaches (BLAST, etc.) to identify potential lanthanide acquisition genes in Methylorubrum extorquens AM1.
• Routinely performed DNA extractions from bacterial samples for Next Gen Sequencing and cloning experiments.
• Developed and assessed a series of 9 bacterial mutants through molecular techniques, such as DNA extraction and cloning to assess phenotypic changes in lanthanide acquisition and storage.
• Developed an optimized staining method that allowed for the identification of lanthanide acquisition in genetic mutants within an hour.
• Created bacterial mutants (gene knockouts/knock-ins) using PCR, PCR primer design, plasmid construction, and cloning.
• Evaluated bacterial mutants to assess qualities such as growth rate and lanthanide acquisition rate using various molecular diagnostic assays such as Arsenazo(III) Assay.

Master'S Degree, Bioinformatics

Bachelor Of Science (Bs), Molecular Biology